GENETIC DIVERSITY OF THE ENVELOPE GLYCOPROTEIN FROM HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ISOLATES OF AFRICAN ORIGIN
GENETIC DIVERSITY OF THE ENVELOPE GLYCOPROTEIN FROM HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ISOLATES OF AFRICAN ORIGIN
复制标题
DOI：
10.1128/jvi.69.1.263-271.1995
复制
发表时间：
1995-01-01
影响因子：
5.4
通讯作者：
BURKE, DS
中科院分区：
文献类型：
--
作者：
LOUWAGIE, J;JANSSENS, W;BURKE, DS
研究了人类免疫缺陷病毒1型（HIV-1）分离株包膜糖蛋白的遗传多样性。HIV-1分离株来自非洲的八个国家：吉布提、加蓬、肯尼亚、塞内加尔、索马里、乌干达、扎伊尔和赞比亚。PCR扩增编码完整HIV-1包膜蛋白的DNA序列并测序。本研究的21个序列和32个以前发表的全长env HIV-1序列之间的系统发育关系进行了确定。20个新测序的非洲分离株可以被分配到包膜亚型A、C、D和G。在赞比亚收集的一个分离株不属于先前描述的8种亚型中的任何一种，可能代表其包膜亚型的原型序列。这些菌株的系统发育分类得到了强有力的支持自助和一致性的树产生的距离方法或最大简约分析。本研究中提供的数据证实了全球HN流行病中存在几种遗传亚型，并扩大了以前观察到的包膜亚型的遗传变异性。不同亚型的地理分布被证明是实质性的，并加强了亚型共循环的概念。
The genetic diversity of the envelope glycoprotein of human immunodeficiency virus type 1 (HIV-1) isolates was studied. HIV-1 isolates were obtained from eight countries in Africa: Djibouti, Gabon, Kenya, Senegal, Somalia, Uganda, Zaire, and Zambia. The DNA sequences encoding the complete HIV-1 envelope protein were PCR amplified and sequenced. Phylogenetic relationships among the 21 sequences from this study and the 32 previously published full-length env HIV-1 sequences were determined. Twenty of the newly sequenced African isolates could be assigned to envelope subtypes A, C, D, and G. One isolate, collected in Zambia, did not belong to any of the eight previously described subtypes and may represent a prototype sequence of its envelope subtype. The phylogenetic classification of these isolates was strongly supported by bootstrapping and the congruence of trees generated by either distance methods or maximum parsimony analysis. The data presented in this study confirm the existence of several genetic subtypes within the global HN epidemic and broaden the genetic variability previously observed for envelope subtypes. The geographic spread of different subtypes was shown to be substantial, and the notion of cocirculation of subtypes was reinforced.