Corticotropin-releasing hormone (Crh) maps to mouse chromosome 3.
Corticotropin-releasing hormone (Crh) maps to mouse chromosome 3.
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促肾上腺皮质激素释放激素 (Crh) 定位于小鼠 3 号染色体。
DOI：
10.1007/bf00361396
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Camper,SA
中科院分区：
文献类型：
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作者：
Knapp,LT;Keegan,CE;Seasholtz,AF;Camper,SA
哺乳动物的应激反应在很大程度上是通过下丘脑-垂体前叶-肾上腺（HPA）轴介导的。该轴中的关键下丘脑释放因子是促肾上腺皮质激素释放激素（CRH）（Vale et al. 1983）。该41-氨基酸肽从正中隆起释放，并通过垂体门静脉血液系统转运至垂体前叶，在垂体前叶增加阿黑皮素原mRNA的合成和促肾上腺皮质激素的分泌（ACTH; Vale et al. 1981）。ACTH刺激肾上腺皮质中糖皮质激素的产生。除了CRH在HPA应激轴中的作用外，CRH还在中枢神经系统内的许多其他区域表达，在这些区域中，CRH被认为影响对应激的行为、自主和免疫反应（Brown and Fisher 1985;欧文等人1992; Koob and Bloom 1985）。通过体细胞杂交的Southern印迹分析，CRH被定位于人染色体（Chr）8，并通过中期染色体的原位杂交定位于带8 q13（Arbiser et al. 1988）。小鼠和人之间的同线性同源性表明Crh将定位于小鼠Chr 3、4、8或15（Nadeau等，1992）。spretus种间回交（C57 BL/6 J-tg 9257· SPRET/Ei）·C57 BL/6 J（Bain et al. 1993;巴罗et al. 1993）。该杂交先前在远端Chr 3、Gba、Hsd 3b、Tshb和Amy-1上的四个位点分型（Bain等，1993）。我们通过对近端Chr 3，碳酸酐酶2（Car-2）和白细胞介素2（II-2）的两个标记进行分型来扩展这种交叉的特征。Southern印迹杂交用于绘制Crh和Car-2图谱，Car-2是一种催化二氧化碳可逆水合反应生成碳酸氢盐的酶（图1; Venta等人，1985）。II '2是一种诱导活化T细胞完成细胞周期的淋巴因子，
The mammalian stress response is mediated in large part via the hypothalamus-anterior pituitary-adrenal (HPA) axis. The key hypothalamic releasing factor in this axis is corticotropin-releasing hormone (CRH)(Vale et al. 1983). This 41-amino-acid peptide is released from the median eminence and transported via the hypophyseal portal blood system to the anterior pituitary, where it increases synthesis of pro-opiomelanocortin mRNA and secretion of adrenocorticotropin (ACTH; Vale et al. 1981). ACTH stimulates the production of glucocorticoids in the adrenal cortex. In addition to the role of CRH within the HPA stress axis, CRH is expressed in many other regions within the central nervous system where it is thought to influence behavioral, autonomic, and immunological responses to stress (Brown and Fisher 1985; Irwin et al. 1992; Koob and Bloom 1985). CRH has been assigned to human Chromosome (Chr) 8 by Southern blot analysis of somatic cell hybrids and localized to band 8q13 by in situ hybridization of metaphase chromosomes (Arbiser et al. 1988). Synteny homology between mice and humans suggested that Crh would map to mouse Chr 3, 4, 8, or 15 (Nadeau et al. 1992).We mapped Crh to mouse Chr 3 using a M. spretus interspecific backcross (C57BL/6J-tg9257• SPRET/Ei)• C57BL/6J (Bain et al. 1993; Barrow et al. 1993). This cross was previously typed at four loci on distal Chr 3, Gba, Hsd3b, Tshb, and Amy-1 (Bain et al. 1993). We extended the characterization of this cross by typing two markers on proximal Chr 3, carbonic anhydrase 2 (Car-2) and interleukin 2 (II-2). Southern blot hybridization was used to map Crh and Car-2, an enzyme that catalyzes the reversible hydration reaction of carbon dioxide to bicarbonate,(Fig. 1; Venta et al. 1985). II'2, a lymphokine that induces activated T-cells to complete the cell cycle, was mapped