Cloning and sequencing of the mouse Gli2 gene: Localization to the Dominant hemimelia critical region
Cloning and sequencing of the mouse Gli2 gene: Localization to the Dominant hemimelia critical region
复制标题
DOI：
10.1006/geno.1996.4468
复制
发表时间：
1997-01-15
期刊：
影响因子：
4.4
通讯作者：
Hill, RE
中科院分区：
文献类型：
--
作者：
Hughes, DC;Allen, J;Hill, RE
锌指基因GLI家族与肿瘤和发育障碍均有关联。我们克隆了小鼠锌指基因GLi2的同源基因，并对其进行了测序，发现该基因与人类的GLI3基因有显著的相似性。我们还将GLi2定位在小鼠1号染色体上，位于形态发生突变显性半指(Dh)附近，其特征是胫骨半缺失，多指/少指，以及一些内脏异常，最显著的是没有脾。利用与Gli2相关的微卫星，我们证明了在Dh种内回交中，Dh和Gli2之间没有重组。GLi2在dh杂合子和纯合子中都有表达，但是通过错配分析和直接测序的方法，我们未能从dh中发现GLi2编码序列的任何突变。我们还证明，在小鼠基因组中，除了先前描述的Gli、Gli2和Gli3之外，不太可能存在任何Gli基因。(C)1997年学术出版社
The GLI family of zinc finger genes has been implicated in both neoplastic and developmental disorders. We have cloned and sequenced the mouse homolog of the zinc finger gene GLi2 and demonstrated significant similarity to the human GLI3 gene. We have also localized GLi2 to mouse chromosome 1, in the vicinity of the morphogenetic mutation Dominant hemimelia (Dh), which is characterized by tibial hemimelia, poly/oligodactyly, and a number of visceral abnormalities, most strikingly absence of the spleen. Using a Gli2-associated microsatellite, we demonstrated no recombination between Dh and GLi2 in a Dh intraspecific backcross. GLi2 is expressed in Dh heterozygotes and homozygotes, However, using a combination of mismatch analysis and direct sequencing, we have failed to identify any mutations in the coding sequence of GLi2 from Dh. We have also demonstrated that it is unlikely that there are any GLi genes in the mouse genome in addition to the previously described GLi, Gli2, and GLi3. (C) 1997 Academic Press