Characterization of two Haemophilus somnus Fc receptors.
Characterization of two Haemophilus somnus Fc receptors.
复制标题
两种睡眠嗜血杆菌 Fc 受体的表征。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
L. Corbeil
中科院分区：
文献类型：
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作者：
M. Yarnall;R. Gogolewski;L. Corbeil
嗜血杆菌表达与牛免疫球蛋白Fc区结合的两种受体，即IgA和IgM。在本研究中，我们对这两种Fc受体之间的关系进行了研究。大分子受体(350、270和120 kDa)分泌到培养基中，也存在于培养基中的不溶蛋白部分。41 kDa Fc受体是一种主要的外膜蛋白，仅存在于不溶蛋白组分中。两种Fc受体的肽图显示，41 kDa受体与高分子质量受体复合体有关。二硫键不太可能是41 kDa受体与高分子质量受体结合的机制，因为还原剂对分离单个受体没有任何作用。虽然41 kDa受体是睡眠嗜血杆菌外膜的主要蛋白质，但在Western blotting中它不与恢复期的牛血清发生反应。相比之下，恢复期的牛血清与Western blotts中的高分子质量受体发生强烈反应。
Haemophilus somnus expresses two types of receptors that bind to the Fc region of bovine IgG, IgA and IgM. In this study, the relationship between these two types of Fc receptors is characterized. The high molecular mass receptors (350, 270 and 120 kDa) were secreted into the culture medium and were also in the insoluble protein fraction of the culture medium. The 41 kDa Fc receptor, which is a major outer-membrane protein, was only present in the insoluble protein fraction. Peptide mapping of the two types of Fc receptors suggests that the 41 kDa receptor is related to the high molecular mass receptor complex. Disulphide linkage is unlikely to be the mechanism of association of the 41 kDa receptor with the high molecular mass receptors since reducing agents had no effect on separating the individual receptors. Although the 41 kDa receptor is a major protein in the outer membrane of H. somnus, it does not react with convalescent bovine sera in Western blots. In contrast, convalescent bovine sera reacts intensely with the high molecular mass receptors in Western blots.
DOI：
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发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Reis,KJ;Ayoub,EM;Boyle,MD
通讯作者：
Boyle,MD