An insertion-deletion at a pentatricopeptide repeat locus linked to fertility transition to cytoplasmic male sterility in radish (Raphanus sativus L.)
An insertion-deletion at a pentatricopeptide repeat locus linked to fertility transition to cytoplasmic male sterility in radish (Raphanus sativus L.)
复制标题
五肽重复位点的插入删除与萝卜（Raphanus sativus L.）的育性转变为细胞质雄性不育有关
DOI：
10.1007/s11032-015-0301-x
复制
发表时间：
2015
影响因子：
3.1
通讯作者：
Ting Wang
中科院分区：
文献类型：
--
作者：
Zhiwei Wang;Chu;e Wang;Shiyong Mei;Lei gao;Yuan Zhou;Ting Wang
细胞质雄性不育（CMS）/细胞核控制育性恢复（Rf）系统已广泛应用于杂交种子生产，是剖析细胞核与线粒体相互作用的理想模型。在这项研究中，我们在萝卜 (Raphanus sativusL.) 的 CMS 雄性生育力恢复位点 (RsRf3) 上发现了一个插入删除 (InDel)。发现了编码五肽重复序列 (PPR) 的等位基因 RsRf3-4，它编码长度为 579 个氨基酸的预测蛋白，包含 14 个 PPR 结构域并具有假定的线粒体靶向信号。序列比对显示，RsRf3-3和RsRf3-4之间存在长度为26 bp的InDel和两个点突变。与RsRf3-4相比，该缺失改变了阅读框，导致RsRf3-3的氨基酸序列被截短。杂交试验表明，RsRf3-3 等位基因不能恢复萝卜 CMS 的育性。相反，RsRf3-4等位基因在分离群体中与CMS的生育力恢复共分离。这些结果强烈表明，RsRf3 基因座的 InDel 可能涉及 CMS 萝卜向育性的转变。
The cytoplasmic male sterility (CMS)/nucleus-controlled fertility restoration (Rf) system has been widely applied in hybrid seed production and is an ideal model for dissecting the interaction between the nucleus and the mitochondrion. In this study, we identified an insertion–deletion (InDel) at a male fertility restoration locus (RsRf3) for CMS in radish (Raphanus sativusL.). A pentatricopeptide repeat (PPR)-encoding alleleRsRf3-4was found and encodes a predicted protein 579 amino acids in length, comprising 14 PPR domains and with a putative mitochondrial targeting signal. Sequence alignment revealed an InDel 26 bp in length and two point mutations betweenRsRf3-3andRsRf3-4.Compared withRsRf3-4, the deletion changed the reading frame, resulting in a truncated amino acid sequence inRsRf3-3. A crossing test showed that theRsRf3-3allele did not restore fertility to CMS in radish. In contrast, theRsRf3-4allele cosegregated with fertility restoration to CMS in a segregation population. These results strongly suggest that the InDel at theRsRf3locus could involve transition to fertility in CMS radish.