Identification of plasmolipin as a major constituent of white matter clathrin-coated vesicles.
Identification of plasmolipin as a major constituent of white matter clathrin-coated vesicles.
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鉴定血浆脂蛋白是白质网格蛋白包被的囊泡的主要成分。
DOI：
10.1111/j.1471-4159.1992.tb11352.x
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发表时间：
1992
影响因子：
4.7
通讯作者：
Beard,ME
中科院分区：
文献类型：
--
作者：
Sapirstein,VS;Nolan,CE;Stern,R;Gray-Board,G;Beard,ME
我们已经从牛白色物质中分离出了包被囊泡，并将其与从灰质中分离出的囊泡进行了比较。在白色物质包被的囊泡中几乎不存在突触囊泡抗原，这表明它们与灰质中发现的那些和来自突触膜的囊泡不同。白色物质包被的囊泡也缺乏致密的髓磷脂成分，例如，髓鞘蛋白脂质、半乳糖苷和硫苷脂，以及轴膜周围髓鞘标记物髓鞘相关糖蛋白。另一方面，这些囊泡含有2′，3 ′-环核苷酸磷酸水解酶。囊泡还含有高水平的浆磷脂，一种存在于髓鞘和少突胶质细胞中的蛋白质。发现血浆磷脂在白色物质包被的囊泡中比在灰质包被的囊泡中高四到五倍。基于蛋白质印迹定量，在白色物质包被的囊泡中的浆磷脂浓度是囊泡双层蛋白的3-4%。这些研究表明，一个显着比例的包被囊泡从白色的问题可能是来自独特的膜结构域的髓鞘复合物或少突胶质细胞膜，这是丰富的浆磷脂。
We have isolated and characterized coated vesicles from bovine white matter and compared them to those isolated from gray matter. The virtual absence of synaptic vesicle antigens in the white matter coated vesicles indicates they are distinct from those found in gray matter and from vesicles derived from synaptic membranes. The white matter coated vesicles also lack compact myelin components, e.g., the myelin proteolipid, galactocerebroside, and sulfatides, as well as the periaxolemmal myelin marker myelin‐associated glycoprotein. On the other hand, these vesicles contain 2′,3′‐cyclic nucleotide phosphohydrolase. The vesicles also contain high levels of plasmolipin, a protein present in myelin and oligodendrocytes. Plasmolipin was found to be four to five times higher in white matter coated vesicles than in gray matter coated vesicles. Based on western blot quantitation, the concentration of plasmolipin in white matter coated vesicles is 3–4% of the vesicle bilayer protein. These studies indicate that a significant proportion of coated vesicles from white matter may be derived from unique membrane domains of the myelin complex or oligodendroglial membrane, which are enriched in plasmolipin.