The expression of SOX11, cyclin D1, cyclin D2, and cyclin D3 in B-cell lymphocytic proliferative diseases
The expression of SOX11, cyclin D1, cyclin D2, and cyclin D3 in B-cell lymphocytic proliferative diseases
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SOX11、cyclin D1、cyclin D2 和 cyclin D3 在 B 细胞淋巴细胞增殖性疾病中的表达
DOI：
10.1007/s12032-011-9937-5
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发表时间：
2012-06-01
期刊：
影响因子：
3.4
通讯作者：
Li, Jian-Yong
中科院分区：
文献类型：
--
作者：
Cao, Xin;Fan, Lei;Li, Jian-Yong
Sox11主要与胚胎神经发生和组织重塑相关。D细胞周期蛋白(细胞周期蛋白D1、细胞周期蛋白D2和细胞周期蛋白D3)参与细胞转化。采用定量RT-PCR方法检测152例B细胞增生性疾病(B-LPD)组织中Sox11、细胞周期蛋白D1、细胞周期蛋白D2和细胞周期蛋白D3的表达，并用免疫组织化学方法检测15例B细胞增生性疾病患者中Sox11蛋白的表达，以探讨这四种基因在B-LPD中的临床意义。资料显示套细胞淋巴瘤(MCL)组织中Sox11和细胞周期蛋白D1的转录水平高于慢性淋巴细胞白血病(CLL)、弥漫性大B细胞淋巴瘤(DLBCL)、毛细胞白血病(HCL)、脾边缘带淋巴瘤(SMZL)和健康对照组。DLBCL中细胞周期蛋白D1和D2的表达水平均高于SMZL。这四个基因的表达水平之间高度相关。4例MCL中3例SOX11核染色阳性，11例B-LPD核染色阴性。此外，我们还发现，ZAP70阳性的CLL患者的Sox11表达水平高于ZAP70阴性的CLL患者。提示MCL患者有较高水平的Sox11和细胞周期蛋白D1mRNA的表达，特别是核Sox11蛋白的表达。
SOX11 is mainly correlated with embryo neurogenesis and remodeling of tissues. D cyclins (cyclin D1, cyclin D2, and cyclin D3) work in cell transformation. We assessed the expression of SOX11, cyclin D1, cyclin D2, and cyclin D3 mRNA in 152 patients with B-cell lymphocytic proliferative diseases (B-LPD) using qRT-PCR and we detected SOX11 protein using immunohistochemistry in 15 B-LPD patients, to clarify the clinical significance of the four genes in B-LPD. Data showed the transcriptional levels of SOX11 and cyclin D1 were higher for the mantle cell lymphoma (MCL) samples compared with chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), hairy cell leukemia (HCL), splenic marginal zone lymphoma (SMZL), and healthy collators. The expression levels of cyclin D1 and cyclin D2 were both higher in DLBCL than in SMZL. The expression levels of the four genes were highly related to each other. Three of 4 MCL patients showed nuclear staining for SOX11, while other 11 B-LPD examples were negative. Furthermore, we also found the ZAP70-positive CLL patients had higher SOX11 expression levels than ZAP70-negative CLL patients. It was revealed that MCL patients have higher expression levels of SOX11 and cyclin D1 mRNA, specially expressed nuclear SOX11 protein.