The Escherichia Coli Hfq Protein: An Unattended DNA-Transactions Regulator.
The Escherichia Coli Hfq Protein: An Unattended DNA-Transactions Regulator.
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DOI：
10.3389/fmolb.2016.00036
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发表时间：
2016
影响因子：
5
通讯作者：
Węgrzyn G
中科院分区：
文献类型：
--
作者：
Cech GM;Szalewska-Pałasz A;Kubiak K;Malabirade A;Grange W;Arluison V;Węgrzyn G
在大肠杆菌中发现Hfq蛋白作为噬菌体Qβ RNA复制的宿主因子。随后的研究表明，Hfq是细菌基因表达的多效性调节剂。Hfq的调节作用主要归因于其作为RNA伴侣的功能，促进细菌非编码RNA与其mRNA靶标之间的相互作用。因此，它调节mRNA的翻译和稳定性。然而，Hfq也能够与DNA相互作用。它在调节DNA相关过程中的作用已得到证实。在这篇小型综述中，讨论了Hfq如何与DNA相互作用，以及这种蛋白质在调节DNA交易中的作用。特别是，Hfq已被证明参与控制ColE1质粒DNA复制，转座，也可能转录。这些Hfq介导的法规的可能机制进行了描述和讨论。
The Hfq protein was discovered in Escherichia coli as a host factor for bacteriophage Qβ RNA replication. Subsequent studies indicated that Hfq is a pleiotropic regulator of bacterial gene expression. The regulatory role of Hfq is ascribed mainly to its function as an RNA-chaperone, facilitating interactions between bacterial non-coding RNA and its mRNA target. Thus, it modulates mRNA translation and stability. Nevertheless, Hfq is able to interact with DNA as well. Its role in the regulation of DNA-related processes has been demonstrated. In this mini-review, it is discussed how Hfq interacts with DNA and what is the role of this protein in regulation of DNA transactions. Particularly, Hfq has been demonstrated to be involved in the control of ColE1 plasmid DNA replication, transposition, and possibly also transcription. Possible mechanisms of these Hfq-mediated regulations are described and discussed.