Characterization of transcript levels for matrix molecules and proteases in ruptured human anterior cruciate ligaments
Characterization of transcript levels for matrix molecules and proteases in ruptured human anterior cruciate ligaments
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DOI：
10.1080/03008200590935556
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发表时间：
2005-01-01
影响因子：
2.9
通讯作者：
Altman, GH
中科院分区：
文献类型：
--
作者：
Bramono, DS;Richmond, JC;Altman, GH
提高对正常前交叉韧带（ACL）功能或修复过程中细胞反应的理解对于临床评估、理解韧带生物学和实施组织工程策略至关重要。本研究利用定量实时RT-PCR结合单变量和多变量统计分析，建立了一个定量数据库的标记转录表达，可以提供一个“蓝图”的ACL伤口愈合。选择的标志物（I型和III型胶原蛋白，双糖蛋白聚糖，核心蛋白聚糖，MMP-1，MMP-2，MMP-9和TIMP-1）进行了评估，从33撕裂的修复手术中收获。观察到损伤后时期和标记物表达之间的趋势。标记物表达之间存在显著相关性，并且在I型和III型胶原之间最为突出。典型相关分析建立了患者人口统计学和所有标志物表达的组合之间的关系。目前观察到的趋势和相关性可以帮助识别适当的组织样品，并提供标记物表达水平的基线信息，其可以支持韧带组织工程应用的环境线索的体外优化。
An improved understanding of cellular responses during normal anterior cruciate ligament (ACL) function or repair is essential for clinical assessments, understanding ligament biology, and the implementation of tissue engineering strategies. The present study utilized quantitative real-time RT-PCR combined with univariate and multivariate statistical analyses to establish a quantitative database of marker transcript expression that can provide a "blueprint" of ACL wound healing. Selected markers ( collagen types I and III, biglycan, decorin, MMP-1, MMP-2, MMP-9, and TIMP-1) were assessed from 33 torn ACLs harvested during reconstructive surgery. Trends were observed between postinjury period and marker expressions. Significant correlations between marker expression existed and were most prominent between collagen types I and III. Canonical correlation analysis established a relationship between patient demographics and a combination of all marker expressions. The currently observed trends and correlations may assist in identifying appropriate tissue samples and provide a baseline information of marker expression level that can support in vitro optimization of environmental cues for ligament tissue engineering application.