Transcriptional regulatory networks in Saccharomyces cerevisiae
Transcriptional regulatory networks in Saccharomyces cerevisiae
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DOI：
10.1126/science.1075090
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发表时间：
2002-10-25
期刊：
影响因子：
56.9
通讯作者：
Young, RA
中科院分区：
文献类型：
--
作者：
Lee, TI;Rinaldi, NJ;Young, RA
我们已经确定了真核生物酿酒酵母中编码的大多数转录调节因子在活细胞中是如何与基因组中的基因相关联的。正如代谢网络图谱描述了细胞完成代谢过程可能使用的潜在途径一样，这种调节因子 - 基因相互作用网络描述了酵母细胞可用于调节全局基因表达程序的潜在途径。我们利用这些信息来识别网络基序，即网络结构的最简单单元，并证明自动化过程可以利用基序来组装转录调控网络结构。我们的研究结果表明，真核细胞的功能通过转录调节因子网络高度相连，这些转录调节因子又对其他转录调节因子进行调节。
We have determined how most of the transcriptional regulators encoded in the eukaryote Saccharomyces cerevisiae associate with genes across the genome in living cells. Just as maps of metabolic networks describe the potential pathways that may be used by a cell to accomplish metabolic processes, this network of regulator-gene interactions describes potential pathways yeast cells can use to regulate global gene expression programs. We use this information to identify network motifs, the simplest units of network architecture, and demonstrate that an automated process can use motifs to assemble a transcriptional regulatory network structure. Our results reveal that eukaryotic cellular functions are highly connected through networks of transcriptional regulators that regulate other transcriptional regulators.