Genetic diversity in tobacco mild green mosaic tobamovirus infecting the wild plant Nicotiana glauca
Genetic diversity in tobacco mild green mosaic tobamovirus infecting the wild plant Nicotiana glauca
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DOI：
10.1006/viro.1996.0463
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发表时间：
1996-09-01
期刊：
影响因子：
3.7
通讯作者：
GarciaArenal, F
中科院分区：
文献类型：
--
作者：
Fraile, A;Malpica, JM;GarciaArenal, F
对来自澳大利亚、加州、西班牙和东地中海盆地的烟草轻绿色花叶病毒（TMGMV）分离物的变异性和遗传结构进行了分析。核糖核酸酶的保护进行了测定53株与6个探针代表85%的TMGMV基因组。结果表明，TMGMV基因组中的保守结构域在不同的地理群体中是不同的。测定了33个TMGMV分离物基因组两个区域的核苷酸序列。核苷酸多样性值小于其他RNA基因组的报告。对于每个种群，遗传多样性与分离株收集区域的大小无关，澳大利亚和西班牙的遗传多样性特别小。多样性值表明人口分化，这是最大的不同的西班牙和澳大利亚人口之间。非同义与同义位置的核苷酸多样性值表明温和的负选择，除了西班牙人口，它给出了证据的积极选择。总体而言，我们的数据表明，该植物病毒的低遗传多样性可能是由于不同的因素，如积极和消极的选择或新的地区或寄主植物的最近定殖。这些因素的强度似乎是不同的TMGMV地理种群。(C)出版社：Academic Press，Inc.
The variability and genetic structure of tobacco mild green mosaic tobamovirus (TMGMV) was analyzed in a collection of isolates from Nicotiana glauca plants from Australia, California, Spain, and the east Mediterranean Basin. Ribonuclease protection was assayed on 53 isolates with six probes representing 85% of the TMGMV genome. Results showed that conserved domains in the TMGMV genome were different for each geographic population. The nucleotide sequence of two regions of the TMGMV genome was determined for 33 isolates. Nucleotide diversity values were smaller than those reported for other RNA genomes. For each population, genetic diversity was not related to the size of the area from which the isolates were collected, and was particularly small for Australia and Spain. Diversity values indicated population differentiation, which was largest between the less diverse Spanish and Australian populations. Nucleotide diversity values for nonsynonymous vs synonymous positions indicate moderate negative selection, except for the Spanish population for which it gave evidence of positive selection. On the whole, our data indicate that the low genetic diversity for this plant virus may he due to different factors such as positive and negative selection or the recent colonization of new areas or host plants. The intensity of these factors appears to be different for the various TMGMV geographical populations. (C) 1996 Academic Press, Inc.