Microbial biogeography along an estuarine salinity gradient: Combined influences of bacterial growth and residence time
Microbial biogeography along an estuarine salinity gradient: Combined influences of bacterial growth and residence time
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DOI：
10.1128/aem.70.3.1494-1505.2004
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发表时间：
2004-03-01
影响因子：
4.4
通讯作者：
Hobbie, JE
中科院分区：
文献类型：
--
作者：
Crump, BC;Hopkinson, CS;Hobbie, JE
浮游细菌群落组成沿着帕克河口和梅岛湾，在东北部马萨诸塞州的盐度梯度的变化，有关的停留时间和细菌群落倍增时间在春季，夏季和秋季。细菌群落组成的特征与PCR扩增的16 S核糖体DNA的变性梯度凝胶电泳（DGGE）。根据细菌产量（[C-14]亮氨酸掺入）和细菌丰度（直接计数）计算平均群落倍增时间。淡水和海洋人口平流进入河口的浮游细菌群落在所有季节的很大一部分。然而，一个独特的河口群落形成在中等盐度在夏季和秋季，当平均倍增时间远远短于水的停留时间，但不是在春季，当倍增时间是类似的停留时间。DGGE条带中DNA的测序表明，大多数条带代表单一的DNA分型，并且来自不同样品的匹配条带代表相同的DNA分型。大多数河流和沿海海洋浮游细菌的成员，共同的淡水和海洋的系统发育集群内的门变形菌门，拟杆菌门，放线菌。河口浮游细菌也属于这些门，但有关的克隆和分离物从几个不同的环境，包括海洋水柱，淡水沉积物和土壤。
Shifts in bacterioplankton community composition along the salinity gradient of the Parker River estuary and Plum Island Sound, in northeastern Massachusetts, were related to residence time and bacterial community doubling time in spring, summer, and fall seasons. Bacterial community composition was characterized with denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S ribosomal DNA. Average community doubling time was calculated from bacterial production ([C-14] leucine incorporation) and bacterial abundance (direct counts). Freshwater and marine populations advected into the estuary represented a large fraction of the bacterioplankton community in all seasons. However, a unique estuarine community formed at intermediate salinities in summer and fall, when average doubling time was much shorter than water residence time, but not in spring, when doubling time was similar to residence time. Sequencing of DNA in DGGE bands demonstrated that most bands represented single phylotypes and that matching bands from different samples represented identical phylotypes. Most river and coastal ocean bacterioplankton were members of common freshwater and marine phylogenetic clusters within the phyla Proteobacteria, Bacteroidetes, and Actinobacteria. Estuarine bacterioplankton also belonged to these phyla but were related to clones and isolates from several different environments, including marine water columns, freshwater sediments, and soil.