BIOLOGICAL REDUCTIVE DECHLORINATION OF TETRACHLOROETHYLENE AND TRICHLOROETHYLENE TO ETHYLENE UNDER METHANOGENIC CONDITIONS
BIOLOGICAL REDUCTIVE DECHLORINATION OF TETRACHLOROETHYLENE AND TRICHLOROETHYLENE TO ETHYLENE UNDER METHANOGENIC CONDITIONS
复制标题
DOI：
10.1128/aem.55.9.2144-2151.1989
复制
发表时间：
1989-09-01
影响因子：
4.4
通讯作者：
GOSSETT, JM
中科院分区：
文献类型：
--
作者：
FREEDMAN, DL;GOSSETT, JM
修复被四氯乙烯(PCE)和三氯乙烯(TCE)污染的地下水的生物工艺只有在转化产物对环境可接受的情况下才能应用。对PCE和TCE降解微生物的浓缩培养的研究提供了证据，证明在产甲烷条件下，混合培养物能够将PCE和TCE完全脱氯为乙烯，这是一种环境可接受的产品。用[14C]PCE进行的放射性示踪研究表明，[14C]乙烯是最终产物；没有观察到显著的14CO2或14CH4的转化。该途径中的限速步骤似乎是将氯乙烯转化为乙烯。为了维持PCE和TCE的还原脱氯，有必要提供一个电子供体；甲醇是最有效的，尽管氢、甲酸盐、乙酸盐和葡萄糖也是有效的。对抑制剂2-溴乙磺酸的研究表明，产甲烷菌在观察到的PCE和TCE的生物转化中起着关键作用。
A biological process for remediation of groundwater contaminated with tetrachloroethylene (PCE) and trichloroethylene (TCE) can only be applied if the transformation products are environmentally acceptable. Studies with enrichment cultures of PCE- and TCE-degrading microorganisms provide evidence that, under methanogenic conditions, mixed cultures are able to completely dechlorinate PCE and TCE to ethylene, a product which is environmentally acceptable. Radiotracer studies with [14C]PCE indicated that [14C]ethylene was the terminal product; significant conversion to 14CO2 or 14CH4 was not observed. The rate-limiting step in the pathway appeared to be conversion of vinyl chloride to ethylene. To sustain reductive dechlorination of PCE and TCE, it was necessary to supply an electron donor; methanol was the most effective, although hydrogen, formate, acetate, and glucose also served. Studies with inhibitor 2-bromoethanesulfonate suggested that methanogens played a key role in the observed biotransformations of PCE and TCE.