Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line
Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line
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DOI：
10.1016/j.jbiosc.2009.09.050
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发表时间：
2010-04-01
影响因子：
2.8
通讯作者：
Ohmori, Hitoshi
中科院分区：
文献类型：
--
作者：
Kajita, Masamichi;Magari, Masaki;Ohmori, Hitoshi
高突变B细胞系DT40可用于抗体筛选和提高抗体的体外亲和力。为了有效地进行亲和成熟，我们建立了一个工程化的DT40系，其免疫球蛋白突变模式可以通过条件抑制XRCC3的表达从基因转换转变为点突变。(C)2009年，日本生物技术学会。版权所有。
A hypermutating B cell line DT40 is useful for screening antibodies and improving affinity of the selected antibodies in vitro. To perform affinity maturation efficiently, we generated an engineered DT40 line whose immunoglobulin mutation pattern can be transformed from gene conversion into point mutation by conditional suppression of XRCC3 expression. (C) 2009, The Society for Biotechnology, japan. All rights reserved.