Genome and transcriptome of Selaginella kraussiana reveal evolution of root apical meristems in vascular plants
Genome and transcriptome of Selaginella kraussiana reveal evolution of root apical meristems in vascular plants
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DOI：
10.1016/j.cub.2023.08.061
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发表时间：
2023-09
期刊：
影响因子：
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通讯作者：
Wu Liu;Gui Cai;Ning Zhai;Hua Wang;Tengfei Tang;Yuyun Zhang;Zhiyao Zhang;Lijun Sun;
中科院分区：
文献类型：
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作者：
Wu Liu;Gui Cai;Ning Zhai;Hua Wang;Tengfei Tang;Yuyun Zhang;Zhiyao Zhang;Lijun Sun;
根的进化使维管植物能够适应陆地环境。化石证据表明，根在真叶植物（蕨类植物和种子植物）和石松植物（现存维管植物的两个谱系）中独立进化。基于石松植物卷柏的高质量基因组组装、mRNA测序（mRNA-seq）数据和单细胞RNA-seq数据，我们表明石松植物和真叶植物的两个根起源事件在根尖分生组织（RAM）发育的调控中采用了部分相似的分子模块。 InS。 kraussiana，RAM 在生长素的引导下从根茎原基开始，并通过二分分支进行自我复制。生长素信号通路直接上调seuAINTEGUMENTAb(SkeuANTb)，然后SkeuANTb直接促进SkeuANTa和WUSCHEL相关HOMEOBOX13b(SkWOX13b)的表达以维持RAM，部分类似于种子植物拟南芥根起始中涉及euANT分支PLETHORA(AtPLT)基因和AtWOX5的分子途径拟南芥。其他分子模块，例如 SHORT-ROOT 和 SCARECROW，在 S 的 RAM 中也具有部分相似的表达模式。 kraussianaandA. 拟南芥。总的来说，我们的研究不仅提供了S的基因组和转录组工具。 kraussianabut 还指出了在石松植物和真叶植物的根起源过程中 RAM 中一些常见分子模块的使用。
The evolution of roots allowed vascular plants to adapt to land environments. Fossil evidence indicates that roots evolved independently in euphyllophytes (ferns and seed plants) and lycophytes, the two lineages of extant vascular plants. Based on a high-quality genome assembly, mRNA sequencing (mRNA-seq) data, and single-cell RNA-seq data for the lycophyteSelaginella kraussiana, we show that the two root origin events in lycophytes and euphyllophytes adopted partially similar molecular modules in the regulation of root apical meristem (RAM) development. InS. kraussiana, the RAM initiates from the rhizophore primordium guided by auxin and duplicates itself by dichotomous branching. The auxin signaling pathway directly upregulateseuAINTEGUMENTAb(SkeuANTb), and then SkeuANTb directly promotes the expression ofSkeuANTaand theWUSCHEL-RELATED HOMEOBOX13b(SkWOX13b) for RAM maintenance, partially similar to the molecular pathway involving the euANT-branchPLETHORA(AtPLT) genes andAtWOX5in root initiation in the seed plantArabidopsis thaliana. Other molecular modules, e.g.,SHORT-ROOTandSCARECROW, also have partially similar expression patterns in the RAMs ofS. kraussianaandA. thaliana. Overall, our study not only provides genome and transcriptome tools ofS. kraussianabut also indicates the employment of some common molecular modules in RAMs during root origins in lycophytes and euphyllophytes.