Effect of lotus seed resistant starch on tolerance of mice fecal microbiota to bile salt
Effect of lotus seed resistant starch on tolerance of mice fecal microbiota to bile salt
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DOI：
10.1016/j.ijbiomac.2020.02.197
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发表时间：
2020-05-15
影响因子：
8.2
通讯作者：
Zeng, Hongliang
中科院分区：
文献类型：
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作者：
Lei, Suzhen;Li, Xin;Zeng, Hongliang
我们通过培养微生物来研究莲子抗性淀粉（LRS）对小鼠粪便微生物群对胆盐耐受性的影响，并与菊粉（INU）、葡萄糖（GLU）和蜡质玉米淀粉（WAX）进行比较。0.03%~ 0.03%胆盐处理使IRS和INU组的操作分类单位（OTU）和多样性指数增加，GLU组的OTU和多样性指数降低，WAX组的OTU和多样性指数降低。具体地说，LRS促进了潜在使用胆汁酸的乳酸杆菌的增殖，并抑制了潜在有害细菌肠球菌和葡萄球菌的生长。胆盐浓度为1.5%时，GLU、LRS和INU组乳酸菌与盐球菌和颗粒菌呈负相关。随着LRS，氨基酸代谢途径增加，而导致某些疾病的病原体减少。LRS通过促进利用胆汁酸的细菌的增殖和抑制有害细菌的生长来增加小鼠粪便微生物群对胆汁盐的耐受性。(C)2020爱思唯尔B. V.保留所有权利。
We investigated the effect of lotus seed resistant starch (LRS) on mice fecal microbiota tolerance to bile salt by culturing organisms compared to inulin (INU) glucose (GLU) and waxy corn starch (WAX). Operational taxonomic units (OTUs) and diversity indices in IRS and INU groups were increased in the presence of 0.03% to 03% bile salt, while they were decreased in GLU, and OTUs were decreased in WAX. Specifically, LRS promoted proliferation of Lactobacillus, which potentially used bile acid, and inhibited growth of the potentially harmful bacteria Enterococcus and Staphylococcus. Moreover, Lactobacillus was negatively correlated with Salinicoccus and Granulicalella in GLU, LRS and INU groups at 1.5% bile salt. With LRS, amino acid metabolic pathways were increased while pathogens causing certain diseases were decreased. LRS increased the tolerance of mice fecal microbiota to bile salt by promoting the proliferation of bacteria utilizing bile acid and inhibiting the growth of harmful bacteria. (C) 2020 Elsevier B.V. All rights reserved.