Functional analysis of endo-1,4-ß-glucanases in response to Botrytis cinerea and Pseudomonas syringae reveals their involvement in plant-pathogen interactions.
Functional analysis of endo-1,4-ß-glucanases in response to Botrytis cinerea and Pseudomonas syringae reveals their involvement in plant-pathogen interactions.
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对灰霉病菌和丁香假单胞菌反应的内切 1,4-α-葡聚糖酶的功能分析揭示了它们参与植物-病原体相互作用。
DOI：
10.1111/j.1438-8677.2012.00701.x
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Finiti I
中科院分区：
文献类型：
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作者：
Finiti I
植物细胞壁修饰是植物逆境响应的重要组成部分。内切-1，4-β-葡聚糖酶（EG）参与细胞壁编辑过程，例如延伸、成熟和裂解。本文研究了EGs受损的番茄和拟南芥的感染反应。转TomCel 1和TomCel 2反义基因番茄植株对假单胞菌侵染表现出更高的敏感性、愈伤组织引发和茉莉酸途径标记基因表达增加。这两个EGs可能是抗性因子，并可能通过干扰防御信号网络作为胼胝质沉积的负调节因子。一项对一组用葡萄孢和灰葡萄孢挑战的Eg T-DNA插入突变体的研究表明，缺乏其他EG会干扰感染表型、胼胝质沉积、信号通路标记基因的表达和激素平衡。我们的结论是，缺乏EGs可以改变植物对病原体的反应，通过修改细胞壁的特性和/或干扰信号传导途径，有助于产生适当的信号结果。微阵列数据的分析表明，EGs差异表达后，许多不同的植物病原体的挑战，激素处理和许多非生物胁迫。我们发现了一些耐盐和耐渗透胁迫的突变体。我们的研究结果表明，削弱EGs可以改变植物-病原体相互作用，并可能有助于适当的信号在许多不同的生物和非生物植物胁迫反应的结果。
Plant cell wall modification is a critical component in stress responses. Endo‐1,4‐β‐glucanases (EGs) take part in cell wall editing processes,e.g. elongation, ripening and abscission. Here we studied the infection response ofSolanum lycopersicumandArabidopsis thalianawith impaired EGs. TransgenicTomCel1andTomCel2tomato antisense plants challenged withPseudomonas syringaeshowed higher susceptibility, callose priming and increased jasmonic acid pathway marker gene expression. These two EGs could be resistance factors and may act as negative regulators of callose deposition, probably by interfering with the defence‐signalling network. A study of a set ofArabidopsisEG T‐DNA insertion mutants challenged withP. syringaeandBotrytis cinerearevealed that the lack of other EGs interferes with infection phenotype, callose deposition, expression of signalling pathway marker genes and hormonal balance. We conclude that a lack of EGs could alter plant response to pathogens by modifying the properties of the cell wall and/or interfering with signalling pathways, contributing to generate the appropriate signalling outcomes. Analysis of microarray data demonstrates that EGs are differentially expressed upon many different plant–pathogen challenges, hormone treatments and many abiotic stresses. We found someArabidopsisEG mutants with increased tolerance to osmotic and salt stress. Our results show that impairing EGs can alter plant–pathogen interactions and may contribute to appropriate signalling outcomes in many different biotic and abiotic plant stress responses.