WOX11 and 12 Are Involved in the First-Step Cell Fate Transition during de Novo Root Organogenesis in Arabidopsis
WOX11 and 12 Are Involved in the First-Step Cell Fate Transition during de Novo Root Organogenesis in Arabidopsis
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DOI：
10.1105/tpc.114.122887
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发表时间：
2014-03-01
期刊：
影响因子：
11.6
通讯作者：
Xu, Lin
中科院分区：
文献类型：
--
作者：
Liu, Jingchun;Sheng, Lihong;Xu, Lin
新器官发生是植物受伤或分离的组织或器官再生不定根和不定芽的过程。植物激素在器官发生中起着关键作用，但激素作用导致整个过程中第一步细胞命运转变的机制尚不清楚。利用拟南芥的叶片外植体，我们证明了同源异型盒基因WUSCHEL相关的HOMEOBOX11(WOX11)和WOX12参与了新根器官的发生。WOX11直接对伤害诱导的生长素在原形成层及其周围的最大值做出反应，并与其同系物WOX12冗余地作用于上调横向器官边界DOMAIN16(LBD16)和LBD29，导致第一步细胞命运从叶原形成层或其附近的薄壁组织细胞向根形成层细胞转变。此外，我们的结果表明，新根器官发生和愈伤组织形成在起始时具有相似的机制。
De novo organogenesis is a process through which wounded or detached plant tissues or organs regenerate adventitious roots and shoots. Plant hormones play key roles in de novo organogenesis, whereas the mechanism by which hormonal actions result in the first-step cell fate transition in the whole process is unknown. Using leaf explants of Arabidopsis thaliana, we show that the homeobox genes WUSCHEL RELATED HOMEOBOX11 (WOX11) and WOX12 are involved in de novo root organogenesis. WOX11 directly responds to a wounding-induced auxin maximum in and surrounding the procambium and acts redundantly with its homolog WOX12 to upregulate LATERAL ORGAN BOUNDARIES DOMAIN16 (LBD16) and LBD29, resulting in the first-step cell fate transition from a leaf procambium or its nearby parenchyma cell to a root founder cell. In addition, our results suggest that de novo root organogenesis and callus formation share a similar mechanism at initiation.