Unique Tyr-heme double cross-links in F43Y/T67R myoglobin: an artificial enzyme with a peroxidase activity comparable to that of native peroxidases
Unique Tyr-heme double cross-links in F43Y/T67R myoglobin: an artificial enzyme with a peroxidase activity comparable to that of native peroxidases
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F43Y/T67R 肌红蛋白中独特的酪氨酸-血红素双交联：一种人工酶，其过氧化物酶活性与天然过氧化物酶相当
DOI：
10.1039/c9cc02714a
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发表时间：
2019-06-11
影响因子：
4.9
通讯作者：
Lin, Ying-Wu
中科院分区：
文献类型：
--
作者：
Liu, Can;Yuan, Hong;Lin, Ying-Wu
F43Y/T67R肌红蛋白的x射线晶体结构显示Tyr43和血红素4-乙烯基之间独特的tyr3 -血红素双交联，这代表了血红素蛋白翻译后修饰的一种新方法。此外，由于远端His-Arg对的特征，设计的人工酶表现出与天然过氧化物酶相当的过氧化物酶活性，例如最有效的辣根过氧化物酶。
The X-ray crystal structure of F43Y/T67R myoglobin revealed unique Tyr-heme double cross-links between Tyr43 and the heme 4-vinyl group, which represents a novel post-translational modification of heme proteins. Moreover, with the feature of a distal His-Arg pair, the designed artificial enzyme exhibited a peroxidase activity comparable to that of native peroxidases, such as the most efficient horseradish peroxidase.