Stop codon reassignments in the wild
Stop codon reassignments in the wild
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DOI：
10.1126/science.1250691
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发表时间：
2014-05-23
期刊：
影响因子：
56.9
通讯作者：
Rubin, Edward M.
中科院分区：
文献类型：
--
作者：
Ivanova, Natalia N.;Schwientek, Patrick;Rubin, Edward M.
典型的遗传密码被认为在生命的所有领域都是高度保守的，只有极少数例外。通过扫描5.6万亿碱基对的宏基因组数据的终止密码子重新分配事件，我们检测到在一个相当大的比例>1700环境样品检查的重编码。我们观察到广泛的蛋白石和琥珀终止密码子在噬菌体和蛋白石在细菌中的重新定位。我们的数据表明，噬菌体可以感染具有不同遗传密码的宿主，并基于密码差异表现出噬菌体-宿主拮抗作用。在设计遗传密码改变的工程生物体时，应考虑到环境生物体中遗传密码的丰富性和多样性，以防止与自然物种交换遗传信息。
The canonical genetic code is assumed to be deeply conserved across all domains of life with very few exceptions. By scanning 5.6 trillion base pairs of metagenomic data for stop codon reassignment events, we detected recoding in a substantial fraction of the >1700 environmental samples examined. We observed extensive opal and amber stop codon reassignments in bacteriophages and of opal in bacteria. Our data indicate that bacteriophages can infect hosts with a different genetic code and demonstrate phage-host antagonism based on code differences. The abundance and diversity of genetic codes present in environmental organisms should be considered in the design of engineered organisms with altered genetic codes in order to preclude the exchange of genetic information with naturally occurring species.