Mucosal Immunity Related to FOXP3+ Regulatory T Cells, Th17 Cells and Cytokines in Pediatric Inflammatory Bowel Disease
Mucosal Immunity Related to FOXP3+ Regulatory T Cells, Th17 Cells and Cytokines in Pediatric Inflammatory Bowel Disease
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DOI：
10.3346/jkms.2018.33.e336
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发表时间：
2018-12-24
影响因子：
4.5
通讯作者：
Kang, Ki Soo
中科院分区：
文献类型：
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作者：
Cho, Jinhee;Kim, Sorina;Kang, Ki Soo
背景：探讨儿童炎症性肠病(IBD)与叉头盒P3(FOXP3(+))调节性T(Treg)细胞、辅助性T细胞(Th17)及细胞因子相关的黏膜免疫功能。用抗人FOXP3和抗ROR-Gamma t抗体进行免疫组织化学染色。结果：克罗恩病患者回肠末端固有层Foxp3(+)Treg细胞数显著高于正常对照组(P<0.05)。克罗恩病组回肠末端ROR-Gamma-t(+)T细胞较对照组有升高趋势。血清IL-4(9.6+/-1.5比12.7+/-3.0)、IL-21(14.9+/-1.5比26.4+/-9.1)、IL-33(14.3+/-0.9比19.1+/-5.3)和干扰素-γ(15.2+/-5.9比50.2+/-42.4)均显著低于对照组。而血清IL-6浓度(119.1+/-79.6vs.52.9+/-39.1)明显高于对照组。溃疡性结肠炎患者血清IL-17A(64.2+/-17.2比28.3+/-10.0)和IL-22(37.5+/-8.8比27.2+/-3.7)水平显著高于克罗恩病患者。结论：黏膜免疫分析显示溃疡性结肠炎患者血清FOXP3(+)T细胞增多，而Th17细胞极化和标志性细胞因子水平降低，而溃疡性结肠炎患者血清FOXP3(+)T细胞增多。
Background: We aimed to investigate mucosal immunity related to forkhead box P3 (FOXP3(+)) regulatory T (Treg) cells, T helper 17 (Th17) cells and cytokines in pediatric inflammatory bowel disease (IBD).Methods: Mucosal tissues from terminal ileum and colon and serum samples were collected from twelve children with IBD and seven control children. Immunohistochemical staining was done using anti-human FOXP3 and anti-ROR gamma t antibodies. Serum levels of cytokines were analyzed using a multiplex assay covering interleukin (IL)-1 beta, IL-4, IL-6, IL 10, IL-17A/F, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, interferon (IFN)-gamma, soluble CD40L, and tumor necrosis factor-alpha.Results: FOXP3(+) Treg cells in the lamina propria (LP) of terminal ileum of patients with Crohn's disease were significantly (P < 0.05) higher than those in the healthy controls. ROR gamma t(+) T cells of terminal ileum tended to be higher in Crohn's disease than those in the control. In the multiplex assay, serum concentrations (pg/mL) of IL-4 (9.6 +/- 1.5 vs. 12.7 +/- 3.0), IL-21 (14.9 +/- 1.5 vs. 26.4 +/- 9.1), IL-33 (14.3 +/- 0.9 vs. 19.1 +/- 5.3), and IFN-gamma (15.2 +/- 5.9 vs. 50.2 +/- 42.4) were significantly lower in Crohn's disease than those in the control group. However, serum concentration of IL-6 (119.1 +/- 79.6 vs. 52.9 +/- 39.1) was higher in Crohn's disease than that in the control. Serum concentrations of IL-17A (64.2 +/- 17.2 vs. 28.3 +/- 10.0) and IL-22 (37.5 +/- 8.8 vs. 27.2 +/- 3.7) were significantly higher in ulcerative colitis than those in Crohn's disease.Conclusion: Mucosal immunity analysis showed increased FOXP3(+) T reg cells in the LP with Crohn's disease while Th17 cell polarizing and signature cytokines were decreased in the serum samples of Crohn's disease but increased in ulcerative colitis.