Spatiotemporal, physiological and transcriptomic dynamics of wild jujube seedlings under saline conditions
Spatiotemporal, physiological and transcriptomic dynamics of wild jujube seedlings under saline conditions
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盐渍条件下酸枣幼苗的时空、生理和转录组动态
DOI：
10.1093/treephys/tpad001
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发表时间：
--
期刊：
影响因子：
4
通讯作者：
Jian Huang
中科院分区：
文献类型：
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作者：
Aobing He;Zhibo Ma;Yunfei Li;Chen Huang;Jean Wan Hong Yong;Jian Huang
土壤盐碱化是制约中国红枣生产的主要因素。酸枣(Ziziphus jujuba var.)Spinosa)被广泛用作枣(Z.jujuba)的砧木，以克服盐碱条件。为了了解酸枣在盐分条件下的适应机制，我们对不同盐分处理的酸枣幼苗进行了时空和生理评价，并进行了转录分析。盐处理对生物量、光合作用、K+、Na+积累有剂量和时间效应。盐处理诱导了根和叶片中较高水平的水杨酸，而叶片脱落酸也在8d后升高。差异表达基因的数量随着更高的剂量和更长的盐处理时间而增加；伴随着丰富的基因本体论术语的变化，这表明生理活动发生了变化。基因共表达网络分析确定了与盐诱导的叶、茎和根的变化相关的核心基因集。WRKY/MYB/bHLH家族的氮转运蛋白、钾转运蛋白和一些转录因子被聚为响应盐胁迫的中枢基因，这些基因与升高的N和K+/Na+有关。两个WRKY转录因子基因(ZjWRKY6和ZjWRKY65)的异位过表达通过提高抗氧化酶活性、减少丙二醛积累和维持K+/Na+动态平衡，增强了拟南芥转化体的耐盐性。本研究为研究酸枣在盐胁迫过程中的时空、生理和转录动态提供了证据，并为进一步研究提高酸枣耐盐性提供了可能的基因。
Soil salinity is a major constraint limiting jujube production in China. Wild jujube (Ziziphus jujuba var. spinosa) is widely used as the rootstock of jujube (Z. jujuba) to overcome the saline conditions. To understand the adaptive mechanism in wild jujube under saline conditions, we combined spatiotemporal and physiological assessments with transcriptomic analysis on wild jujube seedlings undergoing various salt treatments. These salt treatments showed dose and duration effects on biomass, photosynthesis, K+ and Na+ accumulation. Salt treatments induced higher levels of salicylic acid in roots and leaves, while foliar abscisic acid was also elevated after 8 days. The number of differential expression genes increased with higher doses and also longer exposure of NaCl treatments; with concomitant changes in the enriched Gene Ontology terms that were indicative of altered physiological activities. Gene co-expression network analysis identified the core gene sets associated with salt-induced changes in leaves, stems, and roots, respectively. The nitrogen transporters, potassium transporters and a few transcription factors belonging to WRKY/MYB/bHLH families were clustered as the hub genes responding to salt treatments, which were related to elevated N and K+/Na+. Ectopic overexpression of two WRKY transcription factor genes (ZjWRKY6 and ZjWRKY65) conferred stronger salt-tolerance in Arabidopsis thaliana transformants by enhancing the activities of antioxidant enzymes, decreasing MDA accumulation, and maintaining K+/Na+ homeostasis. This study provided evidence about the spatiotemporal, physiological and transcriptomic dynamics of wild jujube during salt stress and identified potential genes for further research to improve salt tolerance in jujube.