Combined transcriptomic and proteomic analysis of the antibacterial mechanisms of an antimicrobial substance produced by Lactobacillus paracasei FX-6 against colistin-resistant Escherichia coli
Combined transcriptomic and proteomic analysis of the antibacterial mechanisms of an antimicrobial substance produced by Lactobacillus paracasei FX-6 against colistin-resistant Escherichia coli
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DOI：
10.1016/j.lwt.2022.114394
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发表时间：
2023-01-02
影响因子：
6
通讯作者：
Liu，Guo
中科院分区：
文献类型：
--
作者：
Zhang，Huiting;Chen，Cong;Liu，Guo
在食源性致病菌中，质粒介导的移动粘菌素耐药性的出现和传播威胁着食品安全和人类健康，迫切需要开发新型抗菌剂。我们之前的研究表明，副乳杆菌FX-6产生的一种抗菌物质(AMS)具有广谱的抗菌活性，可能是一种有前途的候选物质。本研究旨在探讨AMS对粘菌素耐药大肠埃希菌SHP45的抑菌作用及其机制。结果表明，AMS对金黄色葡萄球菌的最小抑菌浓度(MIC)为0.35。ColiSHP45为4.0 mg/mL。流式细胞术显示AMS破坏了细胞膜的结构完整性。通过透射电子显微镜观察，细菌形态遭到严重破坏，细胞质内形成空心区。进一步的转录学和蛋白质组学分析表明，AMS抑制E。ColiSHP45通过共同上调参与膜损伤(FtsH，ClpX)和蛋白质水解(Lon，Dna J，GroEL)的关键基因和蛋白质，导致DNA损伤和代谢紊乱，以及通过抑制营养物质运输和能量代谢来实现。我们的结果为AMS的抗菌作用提供了新的见解。ColiSHP45，提示由副乳杆菌FX-6产生的AMS可能是一种很有前途的抗菌剂，用于控制食品中对粘菌素耐药的病原体。
The emergence and spread of plasmid-mediated mobile colistin resistance in food-borne pathogens threatens food safety and human health, necessitating the development of novel antimicrobial agents. Our previous studies indicate that an antimicrobial substance (AMS) produced byLactobacillus paracaseiFX-6 could be a promising candidate due to its broad-spectrum antimicrobial activities. This study aimed to investigate the antibacterial effects and mechanisms of AMS against colistin-resistantEscherichia coliSHP45. Our results showed that the minimum inhibitory concentration (MIC) of AMS againstE. coliSHP45 was 4.0 mg/mL. Flow cytometry showed that AMS disrupts the structural integrity of the cell membrane. According to transmission electron microscope (TEM) observations, the bacterial morphology was severely damaged, and hollow areas formed in the cytoplasm. Further transcriptomic and proteomic analysis revealed that AMS inhibitsE. coliSHP45 by co-upregulating critical genes and proteins involved in membrane damage (FtsH,ClpX) and protein hydrolysis (Lon,DnaJ,GroEL), causing DNA damage and metabolic disorders, as well as by inhibiting nutrient transport and energy metabolism. Our results provide new insights into the antibacterial effects of AMS againstE. coliSHP45 and suggest that AMS derived fromLactobacillus paracaseiFX-6 may be a promising antimicrobial agent for controlling colistin-resistant pathogens in food.