Cloning, tissue expression, and nutritional regulation of the α-amylase gene in the herbivorous marine teleost Siganus canaliculatus
Cloning, tissue expression, and nutritional regulation of the α-amylase gene in the herbivorous marine teleost Siganus canaliculatus
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DOI：
10.1016/j.aquaculture.2015.12.031
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发表时间：
2016-03
期刊：
影响因子：
4.5
通讯作者：
Dizhi Xie;Shude Xu;Shuqi Wang;C. You;Yuanyou Li
中科院分区：
文献类型：
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作者：
Dizhi Xie;Shude Xu;Shuqi Wang;C. You;Yuanyou Li
α-淀粉酶（α-amylase）是一种糖酶，在碳水化合物代谢中起主要作用。然而，人们对其在草食性海洋硬骨鱼中的分子和生化特征知之甚少。在本研究中，克隆了α-淀粉酶基因，并测定了其在兔鱼Siganus canaliculatus（中国重要的养殖草食性海洋硬骨鱼）中的组织表达。还研究了该基因的营养调节，包括其 mRNA 表达和酶活性。其cDNA全长1903bp，包含1539bp开放阅读框，编码512个氨基酸的多肽，具有α-淀粉酶家族的所有特征。其 mRNA 在肝胰腺、前肠、中肠和后肠中检测到表达，但在消化系统的其他部位未检测到。四个饲料组的兔鱼分别饲喂以下其中一种：生鱼（RF）、配方饲料（FD）、海藻浒苔（EP）或海藻江蓠（GL）。饲养8周后，4个饲料组间肝胰腺、前肠、中肠、后肠α-淀粉酶mRNA表达水平无显着差异，而RF组α-淀粉酶活性显着低于其他饲料组（P<0.001），GL饲喂的鱼中、后肠α-淀粉酶活性显着高于饲喂GL的鱼。 EP（P<0.01）。这些发现表明兔鱼α-淀粉酶合成的营养调节可能发生在转录后水平。这项研究是对来自草食性海洋养殖硬骨鱼的 α-淀粉酶基因的首次表征。相关性声明α-淀粉酶（α-淀粉酶）是一种重要的糖酶，在碳水化合物代谢中发挥着重要作用。本研究首次对草食性海洋养殖硬骨鱼兔鱼α-淀粉酶基因进行克隆和表征，为开发低成本、高效的以海藻为兔鱼饲料成分的配合饲料奠定了基础。所描述的作品尚未发表或正在考虑在其他地方发表。本研究经汕头大学动物实验伦理委员会审查通过。
Alpha-amylase (α-amylase) is a carbohydrase that plays a major role in carbohydrate metabolism. However, little is known about its molecular and biochemical characterisation in herbivorous marine teleosts. In the present study, an α-amylase gene was cloned and its tissue expression was determined in rabbitfishSiganus canaliculatus, a crucial cultured herbivorous marine teleost in China. The nutritional regulation of the gene, including its mRNA expression and enzymatic activity, was also investigated. The full length of its cDNA was 1903 bp, containing a 1539 bp open reading frame encoding a polypeptide of 512 amino acids, which possessed all the characteristic features of the α-amylase family. Its mRNA expression was detected in hepatopancreas, anterior intestine, middle intestine and posterior intestine, but not in other parts of the alimentary system. Four dietary groups of rabbitfish were each fed one of the following: raw fish (RF), formulated diet (FD), seaweedEnteromorpha prolifra(EP), or seaweedGracilarialemaneiformis(GL). After 8 weeks of feeding, the expression level of α-amylase mRNA in hepatopancreas, anterior intestine, middle intestine and posterior intestine showed no significant differences among the four dietary groups, whereas the α-amylase activity in the RF group was significantly lower than that in the other dietary groups (P< 0.001), and significantly higher in the mid and posterior intestine of fish fed GL than those in fish fed with EP (P< 0.01). These findings suggest that the nutritional regulation of α-amylase synthesis in rabbitfish may occur at the posttranscriptional level. This study is the first characterisation of the α-amylase gene from a herbivorous marine culture teleost.Statement of relevanceAlpha-amylase (α-amylase) is an important carbohydrase that plays a major role in carbohydrate metabolism. This study provides the first investigation of cloning and characterisation of α-amylase gene from a herbivorous marine-cultured teleost rabbitfish, and provides a foundation for the development of low-cost and effective formulated feed with seaweed as a dietary ingredient for rabbitfish. The work described has not been published or is under consideration for publication elsewhere. The study was reviewed and approved by the Ethics Committee of Animal Experiments of Shantou University.