Wnt5a-mediated non-canonical Wnt signalling regulates human endothelial cell proliferation and migration
Wnt5a-mediated non-canonical Wnt signalling regulates human endothelial cell proliferation and migration
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DOI：
10.1016/j.bbrc.2007.10.166
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发表时间：
2008-01-11
影响因子：
3.1
通讯作者：
Charnock-Jones, D. Stephen
中科院分区：
文献类型：
--
作者：
Cheng, Ching-wen;Yeh, Ju-ching;Charnock-Jones, D. Stephen
细胞间相互作用是影响血管生成和内皮细胞功能的关键过程之一。WNT信号是通过细胞-细胞相互作用介导的，参与许多发育过程和细胞功能。在这项研究中，我们研究了Wnt5a和非规范的Wnt通路在人内皮细胞中的可能功能。我们发现Wnt5a介导的非规范WRIT信号调节内皮细胞的增殖。使用抗体、siRNA或下游抑制剂阻断这一途径可抑制内皮细胞的增殖、迁移和单层伤口闭合。我们还发现，当内皮细胞被炎性细胞因子的鸡尾酒处理时，WNT5a的mRNA水平上调。我们的发现表明，非规范的WRIT信号在调节内皮细胞生长中发挥作用，并可能在血管生成中发挥作用。(C)2007 Elsevier Inc.保留所有权利。
Cell to cell interaction is one of the key processes effecting angiogenesis and endothelial cell function. Wnt signalling is mediated through cell-cell interaction and is involved in many developmental processes and cellular functions. In this study, we investigated the possible function of Wnt5a and the non-canonical Wnt pathway in human endothelial cells. We found that Wnt5a-mediated non-canonical Writ signalling regulated endothelial cell proliferation. Blocking this pathway using antibody, siRNA or a down-stream inhibitor led to suppression of endothelial cell proliferation, migration, and monolayer wound closure. We also found that the mRNA level of Wnt5a is up-regulated when endothelial cells are treated with a cocktail of inflammatory cytokines. Our findings suggest non-canonical Writ signalling plays a role in regulating endothelial cell growth and possibly in angiogenesis. (c) 2007 Elsevier Inc. All rights reserved.