MUTATIONS ALTERING THE STRUCTURE OF EPIDERMAL GROWTH-FACTOR LIKE CODING SEQUENCES AT THE DROSOPHILA NOTCH LOCUS
MUTATIONS ALTERING THE STRUCTURE OF EPIDERMAL GROWTH-FACTOR LIKE CODING SEQUENCES AT THE DROSOPHILA NOTCH LOCUS
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DOI：
10.1016/0092-8674(87)90123-1
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发表时间：
1987-11-20
期刊：
影响因子：
64.5
通讯作者：
YOUNG, MW
中科院分区：
文献类型：
--
作者：
KELLEY, MR;KIDD, S;YOUNG, MW
果蝇神经源性蛋白Notch主要由表皮生长因子样序列的串联重复拷贝组成。Notch蛋白含有36个类似egf的元素，但没有两个是完全相同的。在本研究中，8个突变与该蛋白的egf同源元件的单个氨基酸替换相关。突变的遗传分析以及突变体和野生型果蝇的DNA比较表明，串联重复的egf样序列之间存在功能分化，重复序列的总数和一致性重复序列的微小变化对野生型Notch蛋白的功能很重要。一组egf样重复序列可能参与功能Notch二聚体或多聚体蛋白的形成。Notch蛋白的第二个区域中的egf样元件似乎控制着它与另一种神经源性位点——分裂增强子（Enhancer of split）产物的相互作用。
The Drosophila neurogenic protein Notch is largely composed of tandemly repeated copies of an epidermal growth factor-like sequence. Notch protein contains 36 EGF-like elements, but no two are identical. In the present study, eight mutations are correlated with single amino acid substitutions in EGF-homologous elements of this protein. Genetic analyses of the mutations and comparisons of DNA from mutant and wild-type flies indicate that differentiation of function exists among the tandemly repeated EGF-like sequences and that the total number of repeats and minor variations from the consensus repeat sequence are important for wild-type Notch protein function. One group of EGF-like repeats may be involved in the formational of function Notch dimers or multimeric proteins. EGF-like elements in a second region of the Notch protein appear to govern its interaction with the product of Enhancer of split, another neurogenic locus of D. melanogaster.