Interleukin-1β induces sialyl Lewis X on hepatocellular carcinoma HuH-7 cells via enhanced expression of ST3Gal IV and FUT VI gene
Interleukin-1β induces sialyl Lewis X on hepatocellular carcinoma HuH-7 cells via enhanced expression of ST3Gal IV and FUT VI gene
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DOI：
10.1016/j.febslet.2006.09.073
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发表时间：
2006-11-13
期刊：
影响因子：
3.5
通讯作者：
Matsumoto, Kojiro
中科院分区：
文献类型：
--
作者：
Higai, Koji;Miyazaki, Noriko;Matsumoto, Kojiro
我们先前已经证明，人肝癌来源的HH-7细胞在白介素1β(IL-1β)刺激下产生α(1)酸性糖蛋白(AGP)，并增加唾液酸路易斯X(SLeX)抗原的数量，但其机制尚不清楚。在这里，我们报告我们对这一机制的调查。抗SLeX抗体结合实验显示，100U/mLIL-1β刺激HH-7细胞48h后，其SLeX表达水平是对照组的2.5倍。此外，在IL-1β刺激后48h，2，3-唾液酸化N-乙酰乳糖胺的表达逐渐增加，这先于SLeX的表达。α2，3-唾液酸基转移酶活性的增加也先于α1，3-岩藻糖基转移酶活性的增加。IL-1β刺激的HH-7细胞ST3Gal IV、FUT IV和FUT VI的mRNA水平在2~4 h开始升高，而FUT VI的mRNA水平在24 h后逐渐升高，IL-1β诱导的HH-7细胞SLeX表达通过基因特异性小干扰RNA被抑制。抗FUT VI和ST3Gal IV，但不抗FUT IV和ST3Gal III。这些结果表明，IL-1β通过增强FUT VI和ST3Gal IV基因的表达而诱导HH-7细胞SLeX的表达。(C)2006年欧洲生化学会联合会。爱思唯尔出版，版权所有。
We previously demonstrated that human hepatocellular carcinoma-derived HuH-7 cells stimulated with interleukin-1 beta (IL-1 beta) produce alpha(1)-acid glycoprotein (AGP) with increased amounts of sialyl Lewis X (sLeX) antigen, although the mechanism remained obscure. Here, we report our investigation of the mechanism. sLeX expression on HuH-7 cells was induced 2.5 times more after 48 h stimulation with 100 U/mL IL-1 beta compared with control, as indicated by anti-sLeX antibody binding. Furthermore, expression of 2,3-sialylated N-acetyllactosamine increased gradually up to 48 h after IL-1 beta stimulation; this preceded the increase in sLeX expression. Increases in alpha 2,3-sialyltransferase activity also preceded increases in alpha 1,3-fucosyltransferase activity. Furthermore, mRNA levels of ST3Gal IV, FUT IV and VI in HuH-7 cells stimulated with IL-1 beta were increased at 2-4 h, while increases in FUT VI mRNA level occurred gradually after 24 h. IL-1 beta-induced sLeX expression on HuH-7 cells was suppressed by transfection of gene-specific small interference RNAs. against FUT VI and ST3Gal IV but not against FUT IV and ST3Gal III. These data results that IL-1 beta induces expression of sLeX on HuH-7 cells by enhanced expression of FUT VI and ST3Gal IV gene. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.