Murine leukemia viruses: induction of macrophage production of granulocyte-macrophage colony-stimulating factor in vitro.
Murine leukemia viruses: induction of macrophage production of granulocyte-macrophage colony-stimulating factor in vitro.
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鼠白血病病毒：体外诱导巨噬细胞产生粒细胞-巨噬细胞集落刺激因子。
DOI：
10.1093/jnci/65.4.841
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
Sakakeeny,MA
中科院分区：
文献类型：
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作者：
Greenberger,JS;Wroble,LM;Sakakeeny,MA
在37° C和7%CO2条件下，在0.3%琼脂中孵育7天后，用亲嗜性小鼠白血病病毒体外感染新鲜分离的N：NIH（S）小鼠骨髓，在50个或更多粒细胞-巨噬细胞（GM）集落和10-49个细胞簇中检测到的细胞数比对照未感染培养物增加。这种效应仅在接种密度高于5.0× 104 cells/ml时观察到，而在从长期骨髓培养中收获的非贴壁细胞经等体积密度梯度离心纯化的GM祖细胞（GM-CFUc）集落形成单位的巨噬细胞耗竭群体中未观察到。与未感染的相比，需要较少的病毒感染的腹腔渗出液巨噬细胞来刺激给定数量的纯化GM-CFUc中相同数量的GM集落和簇。相反，小鼠白血病病毒感染的T淋巴细胞或NIH/3 T3胚胎成纤维细胞不刺激GM-CFUc集落刺激因子（CSF）的释放。用L929或WEHI-3细胞条件培养液浓缩的CSF培养的感染病毒的新鲜分离的全骨髓单细胞悬液，与未感染的骨髓单细胞悬液相比，每1 × 105个细胞产生更多的GM-CFUc集落和GM簇。这一现象表明，对来自病毒感染骨髓的CSF的反应的集落形成细胞可能与对L929或WEHI-3细胞CSF的反应的集落形成细胞不同。数据表明，在体内或长期骨髓培养中逆转录病毒感染后观察到的粒细胞生成增加部分归因于病毒刺激粘附的骨髓基质细胞（包括巨噬细胞）产生CSF。
Infection in vitro of freshly explanted N:NIH(S) mouse bone marrow with ecotropic murine leukemia viruses produced an increase over control uninfected cultures in the 50 or more cell granulocyte-macrophage (GM) colonies and 10–49 cell clusters detected after 7 days of incubation in 0.3% agar at 37° C and 7% CO2. This effect was observed only at plating densities above 5.0×104cells/ml and was not observed with macrophage-depleted populations of colony-forming units of GM progenitor cells (GM-CFUc) purified by isopyknic density gradient centrifugation of nonadherent cells harvested from long-term bone marrow cultures. Fewer virus-infected, compared to uninfected, peritoneal exudate macrophages were required to stimulate the same number of GM colonies and clusters in a given number of purified GM-CFUc. In contrast, murine leukemia virus infection of T-lymphocytes or NIH/3T3 embryo fibroblasts did not stimulate release of GM-CFUc colony-stimulating factor (CSF). Single cell suspensions of virus-infected freshly explanted whole bone marrow grown in CSF concentrated from L929 or WEHI-3 cell-conditioned medium produced more GM-CFUc colonies and GM clusters/1 ×105cells compared to single cell suspensions of uninfected marrow. This phenomenon suggests that the colony-forming cells responding to CSF from virus-infected marrow may have been different from those responding to L929 or WEHI-3 cell CSF. The data indicate that increased granulopoiesis observed following retrovirus infection in vivo or in long-term marrow cultures was attributable in part to virus stimulation of production of CSF by adherent marrow stromal cells including macrophages.