Control of growth hormone synthesis in cultured GH1 cells by 3,5,3'-triiodo-L-thyronine and glucocorticoid agonists and antagonists: studies on the independent and synergistic regulation of the growth hormone response.
Control of growth hormone synthesis in cultured GH1 cells by 3,5,3'-triiodo-L-thyronine and glucocorticoid agonists and antagonists: studies on the independent and synergistic regulation of the growth hormone response.
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3,5,3-三碘-L-甲状腺氨酸和糖皮质激素激动剂和拮抗剂控制培养的 GH1 细胞中生长激素的合成：生长激素反应的独立和协同调节的研究。
DOI：
10.1021/bi00571a025
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发表时间：
1979
期刊：
影响因子：
2.9
通讯作者：
L. Shapiro
中科院分区：
文献类型：
--
作者：
H. Samuels;F. Stanley;L. Shapiro
Herbert H. Samuels，* Frederick Stanley和Lawrence E. Shapiro摘要：我们先前报道了甲状腺激素诱导生长激素合成和mRNA在培养的GH细胞（大鼠垂体细胞系）中被甲状腺激素核受体调节。此外，糖皮质激素对GH细胞中生长激素合成和mRNA的诱导高度依赖于甲状腺激素。在这项研究中，我们使用无血清培养基和糖皮质激素激动剂、拮抗剂和部分激动剂-拮抗剂，进一步确定了生长激素反应的多激素控制。在不添加糖皮质激素的情况下，3,5,3 '-三碘- l -甲状腺原氨酸（l -三碘甲状腺原氨酸）在孵育24小时内诱导生长激素的合成增加4至6倍，到48小时增加到10至12倍。在不添加l -三碘甲状腺原氨酸的情况下，皮质醇或地塞米松诱导的生长激素反应较小，仅在孵育24-48小时后观察到。相比之下，l -三碘甲状腺原氨酸加皮质醇培养的细胞对生长激素的反应比l -三碘甲状腺原氨酸单独培养的细胞高2 - 5倍，并且表现出与甲状腺激素相似的诱导动力学。l -三碘甲状腺原氨酸的生长激素诱导在3.5 nM地塞米松和12 nM皮质醇时增加一半。黄体酮单独在
Herbert H. Samuels,* Frederick Stanley, and Lawrence E. Shapiro abstract: We have previously reported that the thyroid hormone induction of growth hormone synthesis and mRNA in cultured GH, cells, a rat pituitary cell line, is modulated by the thyroid hormone nuclear receptor. In addition, the induction of growth hormonesynthesis and mRNA by glucocorticoid in GH, cells is highly dependent on thyroid hormone. In this study, using serum free defined medium and glucocorticoid agonists, antagonists, and partial agonist-antagonists, we have further defined the multihormonal control of the growth hormone response. Without glucocorticoid, 3, 5, 3'-triiodo-L-thyronine (L-triiodothyronine) induced a fourto sixfold increase in growth hormonesynthesis within 24 h of incubation, and this increased to 10-to 12-fold by 48h. Without L-triiodothyronine, cortisol or dexamethasone induced a small growth hormone response which was only observed after 24-48 h of incubation. In contrast, the growth hormone response of cells incubated with L-triiodothyronine plus cortisol was two-to fivefold greater than L-triiodothyronine alone and demonstrated induction kinetics which paralleled that of thyroid hormone. With L-triiodothyronine a half-maximal increase in growth hormone induction occurred at 3.5 nM dexamethasone and 12 nM cortisol. Progesterone alone in-