Electron tomography of microtubule end-morphologies in C. elegans embryos.
Electron tomography of microtubule end-morphologies in C. elegans embryos.
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DOI：
10.1007/978-1-60327-993-2_8
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发表时间：
2009
影响因子：
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通讯作者：
E. O'Toole;T. Müller-Reichert
中科院分区：
文献类型：
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作者：
E. O'Toole;T. Müller-Reichert
在本章中，我们描述了早期有丝分裂C的制备。elegansembryos的纺锤体微管的端部形态的断层重建。早期胚胎通过高压冷冻和冷冻替代制备，用于Epon/Araldite中的薄层包埋。我们进一步描述了连续切片的有丝分裂纺锤体中微管的数据采集、断层重建和3-D建模。所提出的技术也适用于其他模型系统。
In this chapter we describe the preparation of early mitoticC. elegansembryos for the tomographic reconstruction of end-morphologies of spindle microtubules. Early embryos are prepared by high-pressure freezing and freeze-substitution for thin-layer embedding in Epon/Araldite. We further describe data acquisition, tomographic reconstruction, and 3-D modeling of microtubules in serially sectioned mitotic spindles. The presented techniques are applicable to other model systems.