A High Percentage of Skin Melanoma Cells Expresses SPANX Proteins
A High Percentage of Skin Melanoma Cells Expresses SPANX Proteins
复制标题
DOI：
10.1097/dad.0b013e3181978d6f
复制
发表时间：
2009-04-01
影响因子：
1.1
通讯作者：
Rappazzo, Giancarlo
中科院分区：
文献类型：
--
作者：
Salemi, Michele;Calogero, Aldo E.;Rappazzo, Giancarlo
SPANX（与X染色体核相关的精子蛋白）基因家族在许多肿瘤中都有表达，如黑色素瘤、骨髓瘤、胶质母细胞瘤、乳腺癌、卵巢癌、睾丸生殖细胞肿瘤和血液恶性肿瘤。然而，迄今为止，尚未设计出系统方法来估计表达 SPANX 的癌细胞的百分比。本研究旨在量化黑色素瘤中 SPANX 蛋白的表达。使用我们实验室产生的多克隆抗体，通过免疫组织化学方法评估正常皮肤 (n = 12)、黑色素瘤 (n = 21) 和良性痣 (n = 10) 中 SPANX 蛋白的表达。检查的 21 个黑色素瘤中有 17 个 (80.9%) 表达 SPANX 蛋白。与正常皮肤细胞中没有发现 SPANX 蛋白表达相比，他们的细胞中有很高比例 (49.0% +/- 5.5%) 呈 SPANX 蛋白阳性染色。良性痣的 SPANX 蛋白表达细胞数量处于中等水平（25% +/- 8.5%），其结果显着高于正常皮肤细胞，显着低于皮肤黑色素瘤细胞。在黑色素瘤细胞中，标记大部分位于细胞核，有时不完整或仅限于核周壁，即使在 SPANX 阳性肿瘤细胞中也看到细胞质染色。相比之下，10 个 SPANX 阳性痣中的 5 个具有清晰的信号核定位。这些数据表明 SPANX 蛋白家族在绝大多数测试的黑色素瘤中表达。 LIP SPANX 基因表达的机制以及这些蛋白质的作用尚不清楚。
The expression of SPANX(sperm protein associated with the nucleus in the X chromosome) gene family has been reported in many tumors, such as melanoma, myeloma, glioblastoma, breast carcinoma, ovarian cancer, testicular germ cell tumors, and hematological malignancies. However, no systematic approach has so far been devised to estimate the percentage of cancer cells expressing SPANX. This study was undertaken to quantify the expression of SPANX proteins in melanomas. The expression of SPANX proteins was evaluated by immunohistochemistry in normal skin (n = 12), melanomas (n = 21), and benign nevi (n = 10), using a polyclonal antibody raised in our laboratory. Seventeen of the 21 melanomas (80.9%) examined expressed SPANX proteins. A high percentage of their cells (49.0% +/- 5.5%) stained positively for SPANX proteins compared with no expression found in normal skin cells. Benign nevi had an intermediate number of cells expressing SPANX proteins (25% +/- 8.5%), which resulted significantly higher than normal skin cells and significantly lower than skin melanoma cells. In melanoma cells, the labeling was mostly nuclear, sometimes incomplete or limited to the perinuclear wall, even if cytoplasmic staining was also seen in SPANX-positive tumor cells. In contrast, the 5 of 10 SPANX-positive nevi had a clear nuclear localization of the signal. These data suggest that the SPANX protein family is expressed in the vast majority of the melanomas tested. The mechanism(s), which brings LIP SPANX gene expression and the role of these proteins are not known.