MicroRNA-dependent trans-acting siRNA production.
MicroRNA-dependent trans-acting siRNA production.
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DOI：
10.1126/stke.3002005pe43
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发表时间：
2005-09-06
期刊：
影响因子：
--
通讯作者：
Vaucheret, Herve
中科院分区：
文献类型：
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作者：
Vaucheret, Herve
从在植物中发现反式作用小干扰RNA (tasiRNA) 到阐明相应途径的主要步骤，只用了不到一年的时间。在 tasiRNA 生物发生过程中，从非蛋白质编码 TAS 基因转录的聚腺苷酸化 RNA 被 microRNA (miRNA) 编程的 RNA 诱导沉默复合物裂解。与经典的 miRNA 靶标相比，RDR6 和 SGS3 将 TAS RNA 裂解产物之一转化为双链 RNA，随后在由初始 miRNA 裂解位点确定的阶段中，通过 DICER-LIKE 4 对其进行加工，生成 21 核苷酸的 tasiRNA 群体。 tasiRNA 通过 AGO1 或在某些情况下可能通过 AGO7 的作用引导内源 mRNA 裂解。一些 tasiRNA 靶点可能调节幼年期到成年期的转变，但其他 tasiRNA 靶点的作用仍有待确定。
Less than a year has elapsed between the discovery of trans-acting small interfering RNAs (tasiRNAs) in plants and the elucidation of the major steps of the corresponding pathway. During tasiRNA biogenesis, polyadenylated RNAs transcribed from non-protein-coding TAS genes are cleaved by a microRNA (miRNA)-programmed RNA-induced silencing complex. In contrast to classical miRNA targets, RDR6 and SGS3 convert one of the TAS RNA cleavage products into double-stranded RNA, which is subsequently processed, in a phase determined by the initial miRNA cleavage site, by DICER-LIKE 4 to generate a 21-nucleotide tasiRNA population. tasiRNAs guide endogenous mRNA cleavage through the action of AGO1 or, perhaps in some cases, AGO7. Some of the tasiRNA targets probably regulate the juvenile-to-adult phase transition, but the roles of other tasiRNA targets remain to be determined.