Delineation of Campylobacter concisus genomospecies by amplified fragment length polymorphism analysis and correlation of results with clinical data
Delineation of Campylobacter concisus genomospecies by amplified fragment length polymorphism analysis and correlation of results with clinical data
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DOI：
10.1128/jcm.43.10.5091-5096.2005
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发表时间：
2005-10-01
影响因子：
9.4
通讯作者：
Andersen, LP
中科院分区：
文献类型：
--
作者：
Aabenhus, R;On, SLW;Andersen, LP
与重要的肠道病原体空肠弯曲杆菌一样，简约弯曲杆菌也经常从人类腹泻中分离出来，但它也存在于健康人的粪便中。凝结线虫在人类疾病中的作用一直难以确定，因为该物种至少包括两个表型难以区分但在遗传上截然不同的分类群(即，基因组种)，它们的致病性可能会有所不同。应用扩增片段长度多态(AFLP)技术对62株凝集性C菌进行检测，并与临床资料进行对照。所有菌株都给出了独特的AFLP图谱，这些数据的数值分析将菌株分布在四个聚类群中。该聚类具有分类学意义：两个聚类群分别包含每个已知基因组种的模式菌株(来自口腔)和参考菌株(来自腹泻)。从免疫功能正常的患者和/或无伴发感染的发热、慢性腹泻和肠道炎症的患者中分离到的基因种2菌株比基因种1更常见，与口腔来源的模式菌株聚在一起。出血性腹泻仅记录有致密螺旋体2种感染。我们鉴定了另外两个致密梭菌基因组种：基因组种3包括来自免疫能力强的患者的单个菌株，基因组种4包括来自严重免疫缺陷患者的5个分离株，即器官移植受者或患有血液系统恶性肿瘤的患者。所有4个基因组种都属于相同的蛋白质谱群，不能与基于23S rRNA基因序列的C conisus种特异性聚合酶链式反应：该群的分类地位需要更深入的研究。简约弯曲菌在遗传和分类上是多样化的，至少包含四个不同的基因组种，它们的毒力潜能谱可能存在差异。
Campylobacter concisus has been as frequently isolated from human diarrhea as the important enteropathogen Campylobacter jejuni, but it also occurs in the feces of healthy individuals. The role of C concisus in human disease has been difficult to determine, since the species comprises at least two phenotypically indistinguishable but genetically distinct taxa (i.e., genornospecies) that may vary in pathogenicity. We examined 62 C concisus strains by amplified fragment length polymorphism (AFLP) profiling and correlated the results with clinical data. All C. concisus strains gave unique AFLP profiles, and numerical analysis of these data distributed the strains among four clusters. The clustering was of taxonomic significance: two clusters contained, respectively, the type strain (of oral origin) and a reference strain (from diarrhea) of each of the known genomospecies. Genomospecies 2 strains were more frequently isolated from immunocompetent patients and/or patients without concomitant infections that presented with fever, chronic diarrhea, and gut inflammation than was genomospecies 1, clustering with the type strain of oral origin. Bloody diarrhea was recorded only with C. concisus genomospecies 2 infections. We identified two additional C. concisus genomospecies: genomospecies 3 comprised a single strain from an immunocompetent patient, and genomospecies 4 contained five isolates from severely immunodeficient patients, i.e., organ transplantation recipients or those with hematological malignancies. All genomospecies 4 strains were of the same protein profile group and failed to react with a C concisus species-specific PCR assay based on 23S rRNA gene sequences: the taxonomic position of this group requires closer investigation. Campylobacter concisus is genetically and taxonomically diverse and contains at least four distinct genomospecies that may exhibit differences in their spectra of virulence potential.