A large-scale field study of transgene flow from cultivated rice (Oryza sativa) to common wild rice (O. rufipogon) and barnyard grass (Echinochloa crusgalli).
A large-scale field study of transgene flow from cultivated rice (Oryza sativa) to common wild rice (O. rufipogon) and barnyard grass (Echinochloa crusgalli).
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DOI：
10.1111/j.1467-7652.2006.00210.x
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发表时间：
2006-11
影响因子：
13.8
通讯作者：
Feng Wang;Q. Yuan;Lei Shi;Q. Qian;Wuge Liu;Ba-Geng Kuang;D. Zeng;Y. Liao;B. Cao;S. Jia
中科院分区：
文献类型：
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作者：
Feng Wang;Q. Yuan;Lei Shi;Q. Qian;Wuge Liu;Ba-Geng Kuang;D. Zeng;Y. Liao;B. Cao;S. Jia
转基因作物通过花粉介导的基因流向野生近缘种或杂草的渗入是转基因作物环境风险评估的主要关注点。以转bar基因水稻为花粉供体，普通野生稻为受体，进行了大规模（1.3-1.8公顷）水稻基因流研究。在0-1 m处有高频率的转基因流（11%-18%），随着距离的增加而急剧下降，到250 m时检测限为0.01%。据我们所知，这是转基因水稻到O.目前为止，在此基础上，普通野生稻的原位保护应与常规稻和转基因稻保持足够的隔离距离。同时，没有证据表明转基因渗入稗草，即使它已经与含有bar基因的转基因水稻连续5年共存。因此，基因流到这个杂草物种的环境风险是很少关注的。
The introgression of transgenes into wild relatives or weeds through pollen-mediated gene flow is a major concern in environmental risk assessment of transgenic crops. A large-scale (1.3-1.8 ha) rice gene flow study was conducted using transgenic rice containing the bar gene as a pollen donor and Oryza rufipogon as a recipient. There was a high frequency of transgene flow (11%-18%) at 0-1 m, with a steep decline with increasing distance to a detection limit of 0.01% by 250 m. To our knowledge, this is the highest frequency and longest distance of gene flow from transgenic rice to O. rufipogon reported so far. On the basis of these data, an adequate isolation distance from both conventional and transgenic rice should be taken for in situ conservation of common wild rice. Meanwhile, there is no evidence of transgene introgression into barnyard grass, even when it has coexisted with transgenic rice containing the bar gene for five successive years. Thus, the environmental risk of gene flow to this weedy species is of little concern.