A Super-Assembly of Whi3 Encodes Memory of Deceptive Encounters by Single Cells during Yeast Courtship
A Super-Assembly of Whi3 Encodes Memory of Deceptive Encounters by Single Cells during Yeast Courtship
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DOI：
10.1016/j.cell.2013.10.046
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发表时间：
2013-12-05
期刊：
影响因子：
64.5
通讯作者：
Barral, Yves
中科院分区：
文献类型：
--
作者：
Caudron, Fabrice;Barral, Yves
细胞行为经常受到细胞历史的影响，这表明单个细胞可能会记住过去的事件。我们报告说，芽殖酵母永久逃脱信息素诱导的细胞周期停滞时，经历了欺骗性的交配尝试，即，没有在合理的时间内到达他们的假定伴侣。这种获得性行为依赖于G1/S抑制剂Whi 3的超组装和失活，其将G1细胞周期蛋白Cln 3从翻译抑制中释放出来。Whi 3的超组装是对信息素的缓慢响应，由polyQ和polyN结构域驱动，由Hsp 70抵消，并且在世代中稳定。与朊病毒聚集体不同，Whi 3超组装体不是通过有丝分裂遗传的，而是分离到母细胞中。我们建议，这种polyQ和polyN为基础的元素，这里称为记忆，作为细胞记忆设备编码以前的环境条件。
Cellular behavior is frequently influenced by the cell's history, indicating that single cells may memorize past events. We report that budding yeast permanently escape pheromone-induced cell-cycle arrest when experiencing a deceptive mating attempt, i.e., not reaching their putative partner within reasonable time. This acquired behavior depends on super-assembly and inactivation of the G1/S inhibitor Whi3, which liberates the G1 cyclin Cln3 from translational inhibition. Super-assembly of Whi3 is a slow response to pheromone, driven by polyQ and polyN domains, counteracted by Hsp70, and stable over generations. Unlike prion aggregates, Whi3 super-assemblies are not inherited mitotically but segregate to the mother cell. We propose that such polyQ- and polyN-based elements, termed here mnemons, act as cellular memory devices to encode previous environmental conditions.