Induction of odorant-evoked current transients in Xenopus oocytes injected with mRNA isolated from the olfactory mucosa of Rana pipiens.
Induction of odorant-evoked current transients in Xenopus oocytes injected with mRNA isolated from the olfactory mucosa of Rana pipiens.
复制标题
注射从 Rana pipiens 嗅粘膜分离的 mRNA 的非洲爪蟾卵母细胞中诱导气味诱发的电流瞬变。
DOI：
10.1016/0304-3940(88)90771-9
复制
发表时间：
1988
影响因子：
2.5
通讯作者：
Getchell,TV
中科院分区：
文献类型：
--
作者：
Getchell,TV
Total mRNA isolated from the olfactory mucosa of the frog,Rana pipiens, and from rat pup brains was microinjected into mature oocytes isolated fromXenopus laevis. Both populations of voltage-clamped oocytes as well as controls were non-responsive to an odorant mixture and other ligands two days later. On the 3rd day, oocytes microinjected with rat brain mRNA responded to 5-hydroxytryptamine but not to an odorant mixture, while oocytes injected withRana pipiensolfactory mucosal mRNA responded to an odorant mixture but not to other ligands. The magnitude of the small-amplitude, long-duration inward current transients recorded in response to odorant stimulation was enhanced by preincubating the oocytes with 8-bromo cAMP. The data suggest that odorant-gated ion channels associated with sensory transduction in the primary olfactory system were translated and inserted into the oocyte membrane. The results also indicate that theXenopusoocyte system may be a useful preparation in which to investigate further the expression of membrane events associated with olfactory transduction.
