A low-cost miniaturized potentiostat for point-of-care diagnosis
A low-cost miniaturized potentiostat for point-of-care diagnosis
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DOI：
10.1016/j.bios.2014.06.053
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发表时间：
2014-12-15
影响因子：
12.6
通讯作者：
Bhansali, Shekhar
中科院分区：
文献类型：
--
作者：
Cruz, Andres Felipe Diaz;Norena, Nicolas;Bhansali, Shekhar
本文提出了一种使用小型恒电位仪 (M-P) 芯片 (LMP91000) 进行全范围循环伏安 (CV) 测量以检测生物标志物的新方法。 LMP91000 评估板经过重新配置，可以执行三电极 CV 测量，以实现电化学皮质醇免疫传感。用于皮质醇估计的微电极是通过将单克隆抗皮质醇抗体（Anti-M-Cab）固定到自组装单层（SAM）修饰的金微电极上而制成的。将使用 M-P 获得的结果与使用传统恒电位仪获得的结果进行比较。 M-P 成功测量了 pM 范围内的皮质醇水平。研究结果表明，M-P 可以有效地对三个电极系统进行生化测量，从而能够开发用于即时诊断 (POC) 的微型系统。 (C) 2014 年由 Elsevier B.V. 出版
This paper presents a novel approach of using a miniaturized potentiostat (M-P) chip (LMP91000) to perform full range cyclic voltammetry (CV) measurements for the detection of biomarkers. The LMP91000 evaluation board was reconfigured to perform three-electrode CV measurements in order to achieve electrochemical cortisol immunosensing. The microelectrodes for cortisol estimation were fabricated by immobilizing monoclonal anti-cortisol antibody (Anti-M-Cab) onto self-assembled monolayer (SAM) modified Au microelectrodes. The results obtained using the M-P were compared to those obtained using a conventional potentiostat. The M-P was successful in measuring cortisol levels in the range of pM. The outcomes of the studies suggest that M-P can effectively perform biochemical measurements on three electrode systems, enabling the development of miniature systems for point-of-care (POC) diagnosis. (C) 2014 Published by Elsevier B.V.