Maize Tumors Caused by Ustilago maydis Require Organ-Specific Genes in Host and Pathogen
Maize Tumors Caused by Ustilago maydis Require Organ-Specific Genes in Host and Pathogen
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DOI：
10.1126/science.1185775
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发表时间：
2010-04-02
期刊：
影响因子：
56.9
通讯作者：
Walbot, Virginia
中科院分区：
文献类型：
--
作者：
Skibbe, David S.;Doehlemann, Gunther;Walbot, Virginia
玉米黑粉病（Ustilago maydis）感染玉米提供了一个重要的农艺学模型的生物营养宿主-病原体相互作用。在玉米表皮穿透后，宿主组织的真菌定殖诱导所有气生玉米器官上的肿瘤形成。我们假设不同的原基转化为植物肿瘤需要宿主和病原体的器官特异性基因表达，并通过转录组分析记录了这些差异。对U.缺失编码分泌蛋白基因的玉米突变体和具有器官特异性缺陷的玉米突变体证实了器官限制性肿瘤发生。这是探索单个病原体效应子如何以器官特异性模式部署，与宿主因子相互作用以将正常个体发育重新编程为肿瘤途径的基础。
Infection of maize by corn smut (Ustilago maydis) provides an agronomically important model of biotrophic host-pathogen interactions. After penetration of the maize epidermis, fungal colonization of host tissue induces tumor formation on all aerial maize organs. We hypothesized that transformation of different primordia into plant tumors would require organ-specific gene expression by both host and pathogen and documented these differences by transcriptome profiling. Phenotypic screening of U. maydis mutants deleted for genes encoding secreted proteins and maize mutants with organ-specific defects confirmed organ-restricted tumorigenesis. This is the foundation for exploring how individual pathogen effectors, deployed in an organ-specific pattern, interact with host factors to reprogram normal ontogeny into a tumor pathway.