Role of YidC in folding of polytopic membrane proteins.
Role of YidC in folding of polytopic membrane proteins.
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DOI：
10.1083/jcb.200402067
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发表时间：
2004-04
期刊：
影响因子：
--
通讯作者：
Kaback HR
中科院分区：
文献类型：
--
作者：
Nagamori S;Smirnova IN;Kaback HR
大肠杆菌YidC基因属于保守的Alb 3/Oxa 1/YidC家族，被认为在膜蛋白的生物合成中起重要作用。在这里，我们使用作为一个模型的乳糖通透酶（LacY），一种膜转运蛋白与已知的三维结构，以确定是否YidC在多位膜蛋白插入和/或折叠中发挥作用。在体内和体外转录/翻译/插入系统的实验表明，YidC本身不是插入所必需的，但在LacY的折叠中起着重要的作用。通过使用体外系统和两种针对构象表位的单克隆抗体，显示LacY在YidC耗尽的膜中结合抗体较差。此外，LacY在没有YidC的情况下制备的蛋白脂质体中也折叠不正确。然而，当蛋白脂质体补充有纯化的YidC时，LacY正确折叠。结果表明，YidC发挥了主要作用，在折叠的LacY进入其最终的三级构象，通过相互作用，可能会发生短暂的插入到膜的脂质相。
YidC of Echerichia coli, a member of the conserved Alb3/Oxa1/YidC family, is postulated to be important for biogenesis of membrane proteins. Here, we use as a model the lactose permease (LacY), a membrane transport protein with a known three-dimensional structure, to determine whether YidC plays a role in polytopic membrane protein insertion and/or folding. Experiments in vivo and with an in vitro transcription/translation/insertion system demonstrate that YidC is not necessary for insertion per se, but plays an important role in folding of LacY. By using the in vitro system and two monoclonal antibodies directed against conformational epitopes, LacY is shown to bind the antibodies poorly in YidC-depleted membranes. Moreover, LacY also folds improperly in proteoliposomes prepared without YidC. However, when the proteoliposomes are supplemented with purified YidC, LacY folds correctly. The results indicate that YidC plays a primary role in folding of LacY into its final tertiary conformation via an interaction that likely occurs transiently during insertion into the lipid phase of the membrane.
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影响因子：
3.6
作者：
ITO, K;AKIYAMA, Y
通讯作者：
AKIYAMA, Y
影响因子：
3.5
作者：
Homma, T;Yoshihisa, T;Ito, K
通讯作者：
Ito, K
影响因子：
4.8
作者：
Jiang, FL;Yi, L;Dalbey, RE
通讯作者：
Dalbey, RE
DOI：
10.1073/pnas.79.22.6894
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
CARRASCO, N;TAHARA, SM;KABACK, HR
通讯作者：
KABACK, HR
影响因子：
2.9
作者：
JUNG, K;JUNG, H;KABACK, HR
通讯作者：
KABACK, HR