Antioxidative effect of folate-modified chitosan nanoparticles
Antioxidative effect of folate-modified chitosan nanoparticles
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DOI：
10.1016/s2221-1691(11)60064-6
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发表时间：
2011-02-01
影响因子：
1.7
通讯作者：
Roy, Somenath
中科院分区：
文献类型：
--
作者：
Chakraborty, Subhankari Prasad;Mahapatra, Santanu Kar;Roy, Somenath
目的：探讨羧甲基壳聚糖-2，2‘-乙二氧基双乙胺-叶酸(CMC-EDBE-FA)对尼古丁氧化应激小鼠组织损伤、组织线粒体和血清谷胱甘肽系统的抗氧化作用。方法：通过2，2‘-乙二氧基二乙胺共价键合叶酸标记的羧甲基壳聚糖制备CMC-EDBE-FA。动物分为对照组、烟碱组(1 mg/kg体重/d)、CMC-EDBE-FA(1 mg/kg体重/d)、尼古丁(1 mg/kg体重/d)和CMC-EDBE-FA(1 mg/kg体重/d)，共7天。观察并比较两组大鼠的脂质过氧化水平、氧化谷胱甘肽水平、抗氧化酶水平及DNA损伤情况。结果：与对照组相比，尼古丁处理组大鼠的脂质过氧化反应、氧化谷胱甘肽水平和DNA损伤均显著增加，而CMC-EDBE-FA组的上述指标明显减轻。此外，补充CMC-EDBE-FA可显著改善尼古丁治疗组降低的抗氧化状态。只有CMC-EDBE-FA处理组与对照组相比无明显变化，而是修复了尼古丁处理组的组织损伤。结论：CMC-EDBE-FA是无毒的，可减轻尼古丁的毒性。
Objective: To evaluate the potency of carboxymethyl chitosan-2, 2' ethylenedioxy bis-ethylamine-Folate (CMC-EDBE-FA) on tissue injury, antioxidant status and glutathione system in tissue mitochondria and serum against nicotine-induced oxidative stress in mice. Methods: CMC-EDBE-FA was prepared on basis of carboxymethyl chitosan tagged with folic acid by covalently linkage through 2, 2' ethylenedioxy bis-ethylamine,. Animals were divided into four groups- control, nicotine (1 mg/kg body weight/day), CMC-EDBE-FA (1 mg/kg body weight/day) and nicotine (1 mg/kg body weight/day) and CMC-EDBE-FA (1 mg/kg body weight/day) for 7 days. Levels of lipid peroxidation, oxidized glutathione level, antioxidant enzyme status and DNA damage were observed and compared. Results: The significantly increase of lipid peroxidation, oxidized glutathione levels and DNA damage were observed in nicotine treated group as compared to control group; those were significantly reduced in CMC-EDBE-FA supplemented group. Moreover, significantly, reduced antioxidant status in nicotine treated group was effectively ameliorated by the supplementation of CMC-EDBE-FA. Only CMC-EDBE-FA treated groups shows no significant change as compared to control group; rather than it repairs the tissue damage of nicotine treated group. Conclusions: These findings suggest that CMC-EDBE-FA is non-toxic and ameliorates nicotine-induced toxicity.