Plasmodium falciparum erythrocyte rosetting is mediated by promiscuous lectin-like interactions.
Plasmodium falciparum erythrocyte rosetting is mediated by promiscuous lectin-like interactions.
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DOI：
10.1084/jem.176.5.1311
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发表时间：
1992-11-01
期刊：
影响因子：
--
通讯作者：
Wahlgren M
中科院分区：
文献类型：
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作者：
Carlson J;Wahlgren M
在此，我们描述了一种测定法，该测定法被开发用于定量用羧基荧光素二乙酸酯（C-FDA）标记的正常红细胞（RBC）与玫瑰花形恶性疟原虫感染的RBC的结合。研究了从各种动物物种或人获得的RBC与玫瑰花结恶性疟原虫感染的RBC的不同菌株或克隆的结合。对于所有检测的寄生虫，观察到A/AB或B/AB血型RBC的玫瑰花结的菌株特异性偏好。A、B或AB血型的玫瑰花结结合的亲和力高于O型红细胞，这反映在当给定寄生虫在优选血型的红细胞中生长时，玫瑰花结结合的亲和力更大。恶性疟原虫在O型血红细胞中生长时形成的小尺寸的结节可能与属于O型血而不是A或B型血提供的对脑型疟疾的相对保护有关。A型血寄生虫在O型或B型红细胞中生长时，肝素可完全阻断其寄生，但在A型红细胞中生长时不能阻断其寄生。类似地，当在O型或A型RBC中生长时，比在B型RBC中生长时，偏好B型血的寄生虫更容易被肝素破坏。几种不同的肝素抑制O型红细胞的玫瑰花结，包括两种单糖，它们是肝素的基本成分。在血型A或B RBC中生长的相同寄生虫的玫瑰花结对肝素的敏感性较低，并且仅被A和B血型抗原的末端单核苷酸和三核苷酸、H二糖和岩藻糖特异性抑制。我们的研究结果表明，玫瑰花结是由多种凝集素样相互作用介导的，其使用依赖于寄生虫表型和受体是否存在于宿主细胞上或不。
Herein we describe an assay that was developed to quantitate the binding of normal red blood cells (RBC), labeled with carboxy fluorescein diacetate (C-FDA), to rosetting Plasmodium falciparum- infected RBC. The binding of RBC obtained from various animal species or humans to different strains or clones of rosetting P. falciparum- infected RBC was studied. A strain-specific preference of rosetting was observed for either blood group A/AB or B/AB RBC for all parasites tested. The higher affinity of rosette binding of blood group A, B, or AB vs. O RBC was reflected in larger rosettes when a given parasite was grown in RBC of the preferred blood group. The small size of the rosettes formed when P. falciparum was grown in blood group O RBC may be the in vitro correlate of the relative protection against cerebral malaria afforded by belonging to blood group O rather than to blood group A or B. Rosettes of a blood group A-preferring parasite could be completely disrupted by heparin only when grown in blood group O or B RBC, but not when grown in blood group A RBC. Similarly, the rosettes of a blood group B-preferring parasite could be more easily disrupted by heparin when grown in blood group O or A RBC than when grown in blood group B RBC. Several different saccharides inhibited rosetting of group O RBC, including two monosaccharides that are basic components of heparin. The rosetting of the same parasites grown in blood group A or B RBC was less sensitive to heparin and was specifically inhibited only by the terminal mono- and trisaccharides of the A and the B blood group antigens, the H disaccharide, and fucose. Our results suggest that rosetting is mediated by multiple lectin-like interactions, the usage of which rely on the parasite phenotype and whether the receptors are present on the host cell or not.