Perlecan, a basement membrane-type heparan sulfate proteoglycan, in the enamel organ: Its intraepithelial localization in the stellate reticulum
Perlecan, a basement membrane-type heparan sulfate proteoglycan, in the enamel organ: Its intraepithelial localization in the stellate reticulum
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DOI：
10.1369/jhc.4a6479.2005
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发表时间：
2005-06-01
影响因子：
3.2
通讯作者：
Saku, T
中科院分区：
文献类型：
--
作者：
Ida-Yonemochi, H;Ohshiro, K;Saku, T
以新生和出生后小鼠磨牙、牙釉质器官和牙乳头原代培养细胞为材料，研究了基底膜型硫酸乙酰肝素蛋白多糖在牙胚发育过程中的定位和生物合成，以阐明其在正常牙齿发育中的作用。Perlecan主要分布在釉质器官的细胞间隙以及牙乳头/牙髓或牙囊中。通过原位杂交，Perlecan核心蛋白的mRNA信号在星状网状细胞和牙乳头/牙髓细胞的胞浆中强烈表达，包括成牙本质细胞和牙囊中的成纤维细胞。此外，通过免疫荧光、免疫沉淀和逆转录聚合酶链式反应，证实了釉质器官和牙乳头/牙髓细胞在体外合成了Perlecan核心蛋白。结果表明，Perlecan由牙上皮细胞合成，并聚集在细胞间隙形成特征性的星状网，其功能尚不清楚。
The localization and biosynthesis of perlecan, a basement membrane-type heparan sulfate proteoglycan, were studied in developing tooth germs by using murine molars in neonatal and postnatal stages and primary cultured cells of the enamel organ and dental papilla to demonstrate the role of perlecan in normal odontogenesis. Perlecan was immunolocalized mainly in the intercellular spaces of the enamel organ as well as in the dental papilla/pulp or in the dental follicle. By in situ hybridization, mRNA signals for perlecan core protein were intensely demonstrated in the cytoplasm of stellate reticulum cells and in dental papilla/pulp cells, including odontoblasts and fibroblastic cells in the dental follicle. Furthermore, the in vitro biosyntheses of perlecan core protein by the enamel organ and dental papilla/pulp cells were confirmed by immunofluorescence, immunoprecipitation, and reverse transcriptase-polymerase chain reaction. The results indicate that perlecan is synthesized by the dental epithelial cells and is accumulated in their intercellular spaces to form the characteristic stellate reticulum, whose function is still unknown.