Mycorrhization affects root distribution of Lotus corniculatus and Calamagrostis epigeios in a nutrient poor heterogeneous soil in a rhizotron experiment
Mycorrhization affects root distribution of Lotus corniculatus and Calamagrostis epigeios in a nutrient poor heterogeneous soil in a rhizotron experiment
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DOI：
10.1016/j.rhisph.2017.06.003
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发表时间：
2017-12-01
期刊：
影响因子：
3.7
通讯作者：
Naeth, M. Anne
中科院分区：
文献类型：
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作者：
Boldt-Burisch, Katja;Naeth, M. Anne
植物生长高度依赖于共生伙伴，如丛枝菌根真菌（AMF），提高植物的养分吸收。然而，很少有人知道AMF是否可以有益地影响空间根系分布，特别是在土壤中的养分浓度不均匀。以卢萨申矿区复垦地为研究对象，研究了百脉根（Lotus corniculatus）和小叶章（Calamagrostis epigeios）在不同菌根条件下的根系分布和植物响应。处理为无菌根和菌根L. corniculatus和C.在无菌土壤中和在无菌土壤中与无真菌土壤溶液和在非无菌土壤中的菌根植物。在无菌土壤中，菌根真菌为外源真菌（摩西漏斗菌和Rhizophagus irregularis），而在非无菌土壤中，菌根真菌为内源真菌。植物生长在根管中，在桑迪土壤基质中有粘土粉砂碎片。处理的响应变量为根密度、根和地上部生物量、地上部组织营养浓度、粘土粉砂碎片中的根相对于总根生物量的百分比、菌根定殖和根内真菌碱性磷酸酶（ALP）活性。Corniculatus植物在粘土粉砂碎片中的根质量密度高于土著菌根和非菌根植物，而C. epigeios只有较高的根质量密度在粘土粉砂碎片相对于桑迪土壤只有在外源菌根植物在无菌土壤与土壤溶液。非菌根C.无菌土壤中的地上部生物量与外源菌根处理没有显著差异。最大的拍摄生物量为两种植物，是最高的百分比的根在粘土粉砂碎片相对于总的根系。两种植物的外源菌根植物在无菌土壤和土壤溶液中的地上部磷浓度最高。然而，根内真菌ALP活性最高的土著菌根植物。结果表明，菌根条件可以有很大的影响空间根系发育。其结果是，促进根系生长的条件，导致更多的根在营养丰富的粘土粉砂碎片，从而提高植物利用不均匀分布的土壤养分。
Plant growth can be highly dependent on symbiotic partners, such as arbuscular mycorrhizal fungi (AMF) that improve the plant's nutrient uptake. However, less is known about whether AMF can beneficially influence spatial root distribution, especially in soils with patchy nutrient concentrations. This study was conducted to evaluate root distribution and plant response of Lotus corniculatus and Calamagrostis epigeios in a heterogeneous nutrient poor soil under different mycorrhizal conditions.Heterogeneous soil conditions of a reclamation site in the Lusation mining district, consisting of a nutrient poor sandy soil with embedded large silt-clay fragments, were used for this rhizotron experiment. Treatments were non-mycorrhizal and mycorrhizal L. corniculatus and C. epigeios in sterile soil and in sterile soil with a fungal free soil solution and mycorrhizal plants in a non-sterile soil. Mycorrhizal fungi were exogenous (Funneliformis mosseae and Rhizophagus irregularis) in sterile soil and indigenous mycorrhizal fungi in non-sterile soil. Plants were grown in rhizotrons, with clay-silt fragments in a sandy soil matrix. Treatment response variables were root density, root and shoot biomass, shoot tissue nutrient concentrations, and percentage of roots in clay-silt fragments relative to total root biomass, mycorrhizal colonization and intraradical fungal alkaline phosphatase (ALP) activity.Exogenous mycorrhizal L. corniculatus plants had higher root mass density in clay-silt fragments than indigenous mycorrhizal and non-mycorrhizal plants while C. epigeios had only higher root mass density in clay-silt fragments relative to sandy soil only in exogenous mycorrhizal plants in sterile soil with soil solution. Non-mycorrhizal C. epigeios shoot biomass in sterile soil did not significantly differ from that of exogenous mycorrhizal treatments. The greatest shoot biomass for both plant species, was with the highest percentage of roots in clay-silt fragments relative to the total root system. Exogenous mycorrhizal plants of both species had highest shoot phosphorus concentrations in sterile soil with soil solution. However, intraradical fungal ALP activity was highest in indigenous mycorrhizal plants. The results indicate that mycorrhizal conditions can have a strong influence on spatial root development. The consequences are that conditions, which promote root growth, lead to more roots in nutrient rich clay-silt fragments and thus enhance plant exploitation of heterogeneously distributed soil nutrients.