Comparative pharmacokinetics and tissue distribution profiles of lignan components in normal and hepatic fibrosis rats after oral administration of Fuzheng Huayu recipe
Comparative pharmacokinetics and tissue distribution profiles of lignan components in normal and hepatic fibrosis rats after oral administration of Fuzheng Huayu recipe
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扶正化瘀方口服后正常与肝纤维化大鼠药代动力学及组织分布特征比较。
DOI：
10.1016/j.jep.2015.03.024
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发表时间：
2015-05-26
影响因子：
5.4
通讯作者：
Liu, Cheng-Hai
中科院分区：
文献类型：
--
作者：
Yang, Tao;Liu, Shan;Liu, Cheng-Hai
民族药理学相关性：扶正化瘀方（FZHY）是根据中医治疗肝纤维化的理论而研制的，本研究的目的：探讨肝纤维化病理状态对FZHY中木脂素类成分的药代动力学及组织分布的影响。结果：建立了一种快速、灵敏、简便的高效液相色谱-串联质谱法（UHPLC-MS/MS），可同时测定正常大鼠和肝纤维化大鼠血浆和组织中的6种木脂素类成分。FZHY 15 g/kg灌胃给药后，肝纤维化大鼠体内五味子醇甲（SIA）、五味子醇B（SI B）、五味子醇丙（SIC）、五味子醇甲（SOA）、五味子醇B（SIX）和五味子酯甲（STA）的药代动力学行为发生了显著变化，AUC（0-t）值分别比正常大鼠增加了235.09%、388.44%、223.30%、669.30%、295.08%和267.63%（P
Ethnopharmacological relevance: Fuzheng Huayu recipe (FZHY) is formulated on the basis of Chinese medicine theory in treating liver fibrosis.Aim of the study: To illuminate the influence of the pathological state of liver fibrosis on the pharmacokinetics and tissue distribution profiles of lignan components from FZHY.Materials and methods: Male Wistar rats were randomly divided into normal group and Hepatic fibrosis group (induced by dimethylnitrosamine). Six lignan components were detected and quantified by ultrahigh performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)in the plasma and tissue of normal and hepatic fibrosis rats.Results: A rapid, sensitive and convenient UHPLC-MS/MS method has been developed for the simultaneous determination of six lignan components in different rat biological samples successfully. After oral administration of FZHY at a dose of 15 g/kg, the pharmacokinetic behaviors of schizandrin A (SIA), schizandrin B (SIB), schizandrin C (SIC), schisandrol A (SOA), Schisandrol B (SOB) and schisantherin A (STA) have been significantly changed in hepatic fibrosis rats compared with the normal rats, and their AUC(0-t) values were increased by 235.09%, 388.44%, 223.30%, 669.30%, 295.08% and 267.63% orderly (P