Gene Expression Disruptions of Organism versus Organ in Drosophila Species Hybrids
Gene Expression Disruptions of Organism versus Organ in Drosophila Species Hybrids
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DOI：
10.1371/journal.pone.0003009
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发表时间：
2008-08-20
期刊：
影响因子：
3.7
通讯作者：
Noor, Mohamed A. F.
中科院分区：
文献类型：
--
作者：
Catron, Daniel J.;Noor, Mohamed A. F.
杂种功能障碍，如不育，可能部分是由于基因表达调控的破坏。对拟果蝇进化枝内的杂交种的研究已经报道了基因表达高于或低于在其亲本物种中观察到的表达，并且这种错误表达与多代回交杂交种中的雄性不育有关。然而，这些研究通常检查整个身体而不是睾丸，或者使用不太敏感但全球性的技术进行有限的复制。在这里，我们使用一种新的RNA分离技术，通过实时定量RT-PCR重新检查单个果蝇雄性睾丸和全身的杂交基因表达中断。我们发现两个早期精子发生的成绩单是低表达的混合整体机构，但不是在单独的睾丸检测，而两个后期精子发生的成绩单似乎是低表达的整体机构和睾丸。虽然调查的成绩单的数量是有限的，这些结果提供了一些支持以前的假设，即在这些不育的杂交后代的精子发生途径可能会被破坏后的早期减数分裂阻滞基因的表达。
Hybrid dysfunctions, such as sterility, may result in part from disruptions in the regulation of gene expression. Studies of hybrids within the Drosophila simulans clade have reported genes expressed above or below the expression observed in their parent species, and such misexpression is associated with male sterility in multigenerational backcross hybrids. However, these studies often examined whole bodies rather than testes or had limited replication using less-sensitive but global techniques. Here, we use a new RNA isolation technique to re-examine hybrid gene expression disruptions in both testes and whole bodies from single Drosophila males by real-time quantitative RT-PCR. We find two early-spermatogenesis transcripts are underexpressed in hybrid whole-bodies but not in assays of testes alone, while two late-spermatogenesis transcripts seem to be underexpressed in both whole-bodies and testes alone. Although the number of transcripts surveyed is limited, these results provide some support for a previous hypothesis that the spermatogenesis pathway in these sterile hybrids may be disrupted sometime after the expression of the early meiotic arrest genes.