Quantitative proteomic analysis of histone modifications.
Quantitative proteomic analysis of histone modifications.
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DOI：
10.1021/cr500491u
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发表时间：
2015-03-25
期刊：
影响因子：
62.1
通讯作者：
Zhao Y
中科院分区：
文献类型：
--
作者：
Huang H;Lin S;Garcia BA;Zhao Y
已经提出了组蛋白标记发挥其功能的几种机制，包括通过乙酰化中和电荷来改变核小体的物理性质，导致核小体迁移率增加和高级染色质结构的调节。此外，组蛋白标记通常通过募集下游分子起作用，称为“阅读器”或“效应器”，其特异性识别组蛋白分子或核小体背景下的特定修饰。1c，291这里，定义了相关蛋白质的几个术语。我们希望将组蛋白标记的蛋白质“阅读器”定义为在特定序列背景下直接相互作用并识别特定组蛋白标记的蛋白质;将蛋白质“结合剂”定义为以修饰依赖性或非依赖性方式直接或间接与组蛋白缔合的蛋白质;以及作为特异性结合翻译后修饰的组蛋白底物的蛋白质的“效应物”，并且该结合事件募集相同多肽或复合物内包含的其它活性。因此，“效应器”将组蛋白标记翻译成生物输出。“阅读器”是组蛋白标记的直接“粘合剂”。
Several mechanisms have been proposed for histone marks to exert their functions, including altering the physical properties of nucleosomes by neutralization of charge via acetylation, resulting in increasing nucleosome mobility and modulation of the higher order chromatin structure. 24b In addition, histone marks often act through the recruitment of downstream molecules, referred to as “readers” or “effectors”, which specifically recognize a particular modification in the context of the histone molecule or nucleosomes. 1c, 291 Here, a few terms for the relevant proteins are defined. We would like to define a protein “reader” of a histone marks as a protein that directly interacts and recognizes a specific histone mark in a particular sequence context; a protein “binder” as a protein that either directly or indirectly associates with a histone, in a modification-dependent or-independent manner; and a “effector” as a protein that binds specifically to a posttranslational modified histone substrate, and this binding event recruits other activities contained within the same polypeptide or complex. Thus,“effectors” translate histone marks into biological output. A “reader” is a direct “binder” of a histone mark.