EXPRESSIONS OF CYTOKINE GENES DURING DEVELOPMENT OF AUTOIMMUNE SIALADENITIS IN MRL/LPR MICE
EXPRESSIONS OF CYTOKINE GENES DURING DEVELOPMENT OF AUTOIMMUNE SIALADENITIS IN MRL/LPR MICE
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DOI：
10.1002/eji.1830231002
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发表时间：
1993-10-01
影响因子：
5.4
通讯作者：
HAYASHI, Y
中科院分区：
文献类型：
--
作者：
HAMANO, H;SAITO, I;HAYASHI, Y
通过直接分析从患有自身免疫性唾液腺炎的 MRL/lpr 小鼠唾液腺组织中获得的 RNA，分析体内局部细胞因子基因的表达。通过逆转录酶聚合酶链反应和免疫组织化学分析评估细胞因子基因的表达。 1、2月龄小鼠唾液腺炎性病变发生前即可检测到白细胞介素-1（IL-1）β和肿瘤坏死因子的表达，而MRL/lpr小鼠3月龄典型自身免疫性唾液腺炎发病时即可清晰检测到IL-6 mRNA表达，且随年龄增长而表达上调。这些结果表明这些炎症细胞因子基因的过度表达参与了MRL/lpr小鼠唾液腺器官局部自身免疫的发生和进展。
Local cytokine gene expression in vivo was analyzed by direct analysis of RNA obtained from salivary gland tissues of MRL/lpr mice with autoimmune sialadenitis. The expression of cytokine genes were assessed by the reverse-transcriptase polymerase chain reaction and by immunohistochemical analysis. The expression of interleukin-1(IL-1)beta and tumor necrosis factor was detected before the onset of inflammatory lesions in the salivary glands of mice of 1 or 2 months of age, and IL-6 mRNA expression was clearly detected at the time of onset of typical autoimmune sialadenitis at 3 months of age in MRL/lpr mice, and was up-regulated with advancing age. These results suggest that the overexpression of these inflammatory cytokine genes is involved in the development and progression of organ-localized autoimmunity in the salivary glands of MRL/lpr mice.