Basic fibroblast growth factor is a calcium-mobilizing secretagogue in rat pancreatic acini.
Basic fibroblast growth factor is a calcium-mobilizing secretagogue in rat pancreatic acini.
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碱性成纤维细胞生长因子是大鼠胰腺腺泡中的钙动员促泌剂。
DOI：
10.1016/0006-291x(91)91963-d
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发表时间：
1991
影响因子：
3.1
通讯作者：
Korc,M
中科院分区：
文献类型：
--
作者：
Chandrasekar,B;Korc,M
碱性成纤维细胞生长因子（bFGF）可使大鼠胰腺腺泡内1，4，5-三磷酸肌醇[Ins（1，4，5）P3]水平显著升高，胞浆游离钙[Ca ~（2+）]水平迅速升高。bFGF介导的钙瞬变不依赖于细胞外钙的存在，并取消预处理的腺泡与卡巴胆碱。碱性成纤维细胞生长因子刺激胰腺腺泡中淀粉酶的释放，在一个双相的，剂量依赖性的方式，这种效果被阻断的中和抗碱性成纤维细胞生长因子抗体。在高得多的浓度下，表皮生长因子（EGF），而不是胰岛素样生长因子-I（IGF-I），部分模仿bFGF的一些行动。这些发现表明，bFGF是一种以前未被认识到的钙动员胰腺促分泌素，可能参与胰腺外分泌功能的调节。
Basic fibroblast growth factor (bFGF) induced a marked increase in the levels of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] and a rapid rise in cytosolic free calcium [Ca2+]ilevels in rat pancreatic acini. The bFGF-mediated calcium transient was not dependent on the presence of extracellular calcium, and was abolished by pretreatment of acini with carbachol. bFGF stimulated amylase release in pancreatic acini in a monophasic, dose-dependent manner, and this effect was blocked by neutralizing anti-bFGF antibodies. At much higher concentrations, epidermal growth factor (EGF), but not insulin-like growth factor-I (IGF-I), partially mimicked some of the actions of bFGF. These findings suggest that bFGF is a previously unrecognized calcium-mobilizing pancreatic secretagogue that may participate in the regulation of pancreatic exocrine function.