Stress responses of the fish Nile tilapia subjected to electroshock and social stressors
Stress responses of the fish Nile tilapia subjected to electroshock and social stressors
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DOI：
10.1590/s0100-879x2006001200012
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发表时间：
2006-12-01
影响因子：
2.3
通讯作者：
Volpato, G.L.
中科院分区：
文献类型：
--
作者：
Barreto, R.E.;Volpato, G.L.
测定了36只成年尼罗罗非鱼雄性（标准长度，平均值+/- SD，14.38 +/- 1.31 cm）的血浆皮质醇和葡萄糖水平，并对其进行了电击和社会应激。在鱼适应实验水族箱（1条鱼/水族箱）后5天测定预应激水平。五天后，评估压力对皮质醇和葡萄糖水平的影响。施加以下应激源60分钟：与较大的常驻动物配对（社会应激源），或温和的电击（AC，20 V，15 m-A，100 Hz，每4分钟1分钟）。每个应激源在两个独立的组中进行测试，其中一组在60分钟应激源施加结束后立即量化（T60），另一组在30分钟后量化（T90）。皮质醇和葡萄糖的前应激值在两组之间没有统计学差异。血浆皮质醇水平显着增加，并在电击和社会应激的幅度相似（基础和最终样本的平均值+/- SD为：电击T60 = 65.47 +/- 15.3，177.0 +/- 30.3; T90 = 54.8 +/- 16.0，196.2 +/- 57.8;社会压力T60 = 47.1 +/- 9.0，187.6 +/- 61.7; T90 = 41.6 +/- 8.1，112.3 +/- 26.8）。血浆葡萄糖水平在两个时间点（T60和T90）的电击显着增加，但只有在T90的社会压力。初始和最终平均（+/- SD）值为：电击T60 = 52.5 +/- 9.2，115.0 +/- 15.7; T90 = 35.5 +/- 1.1，146.3 +/- 13.3;社会压力T60 = 54.8 +/- 8.8，84.4 +/- 15.0; T90分别为34.5 +/- 5.6、116.3 +/- 13.6。因此，电休克诱导葡萄糖的增加速度比社会压力更快。此外，皮质醇和葡萄糖之间的显着正相关关系，仅在T90检测到的社会压力。这些结果表明，一个鱼类物种不同的反应，不同的压力，从而表明鱼类的应激反应的特异性的压力。
Plasma cortisol and glucose levels were measured in 36 adult Nile tilapia males, Oreochromis niloticus (standard length, mean +/- SD, 14.38 +/- 1.31 cm), subjected to electroshock and social stressors. Prestressor levels were determined 5 days after the adjustment of the fish to the experimental aquaria (1 fish/aquarium). Five days later, the effects of stressors on both cortisol and glucose levels were assessed. The following stressors were imposed for 60 min: pairing with a larger resident animal (social stressor), or a gentle electroshock (AC, 20 V, 15 m-A, 100 Hz for 1 min every 4 min). Each stressor was tested in two independent groups, one in which stress was quantified immediately after the end of the 60-min stressor imposition (T60) and the other in which stress was quantified 30 min later (T90). Pre-stressor values for cortisol and glucose were not statistically different between groups. Plasma cortisol levels increased significantly and were of similar magnitude for both electroshock and the social stressor (mean +/- SD for basal and final samples were: electroshock T60 = 65.47 +/- 15.3, 177.0 +/- 30.3; T90 = 54.8 +/- 16.0, 196.2 +/- 57.8; social stress T60 = 47.1 +/- 9.0, 187.6 +/- 61.7; T90 = 41.6 +/- 8.1, 112.3 +/- 26.8, respectively). Plasma glucose levels increased significantly for electroshock at both time points (T60 and T90), but only at T90 for the social stressor. Initial and final mean (+/- SD) values are: electroshock T60 = 52.5 +/- 9.2, 115.0 +/- 15.7; T90 = 35.5 +/- 1.1, 146.3 +/- 13.3; social stress T60 = 54.8 +/- 8.8, 84.4 +/- 15.0; T90 = 34.5 +/- 5.6, 116.3 +/- 13.6, respectively. Therefore, electroshock induced an increase in glucose more rapidly than did the social stressor. Furthermore, a significant positive correlation between cortisol and glucose was detected only at T90 for the social stressor. These results indicate that a fish species responds differently to different stressors, thus suggesting specificity of fish stress response to a stressor.