Designing and generating a mouse model: frequently asked questions.
Designing and generating a mouse model: frequently asked questions.
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DOI：
10.7555/jbr.35.20200197
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发表时间：
2021-03-26
影响因子：
2.3
通讯作者：
Ohtsuka M
中科院分区：
文献类型：
--
作者：
Gurumurthy CB;Saunders TL;Ohtsuka M
基因工程小鼠（GEM）模型常用于生物医学研究。生成GEM涉及一套复杂的实验程序，需要复杂的设备和高度熟练的技术人员。由于这些原因，大多数研究机构都建立了集中的核心设施，为研究小组创建定制的GEM。另一方面，当研究人员开始考虑为他们的研究生成GEM时，他们的脑海中出现了几个问题。例如，什么类型的模型对我的研究最有用，我如何设计它们，开发模型的最新技术和工具是什么，以及如何在我的研究中培育GEM。由于在鼠标设计中有几个考虑因素和选择，而且这是一项昂贵且耗时的奋进，因此预先仔细规划可以确保最大的成功机会。在这篇文章中，我们提供了几个常见的问题，当研究人员开始考虑为他们的工作产生小鼠模型（S）出现的简短答案。
Genetically engineered mouse (GEM) models are commonly used in biomedical research. Generating GEMs involve complex set of experimental procedures requiring sophisticated equipment and highly skilled technical staff. Because of these reasons, most research institutes set up centralized core facilities where custom GEMs are created for research groups. Researchers, on the other hand, when they begin thinking about generating GEMs for their research, several questions arise in their minds. For example, what type of model(s) would be best useful for my research, how do I design them, what are the latest technologies and tools available for developing my model(s), and finally how to breed GEMs in my research. As there are several considerations and options in mouse designs, and as it is an expensive and time-consuming endeavor, careful planning upfront can ensure the highest chance of success. In this article, we provide brief answers to several frequently asked questions that arise when researchers begin thinking about generating mouse model(s) for their work.